MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2
MS data file : PRT1311_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:18:13 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,460

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 501–600 (out of 4256)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
230 309.0929 308.0856
231 309.0936 308.0863
232 309.0939 308.0866
233 309.0947 308.0874
234 309.0947 308.0874
235 309.0948 308.0875
236 309.0949 308.0876
237 309.1307 308.1234
238 309.1314 308.1241
239 309.1320 308.1247
240 309.1321 308.1248
241 309.1323 308.1250
242 309.1324 308.1251
243 309.1325 308.1252
244 309.1326 308.1253
245 309.1326 308.1253
246 309.1327 308.1254
247 309.1327 308.1254
248 309.1328 308.1255
249 309.1328 308.1255
250 309.1328 308.1255
251 309.1328 308.1255
252 309.1328 308.1255
253 309.1328 308.1255
254 309.1329 308.1256
255 309.1333 308.1260
256 309.1335 308.1262
257 309.1490 308.1417
258 309.1692 308.1619
259 309.1693 308.1620
260 309.1693 308.1620
261 309.1693 308.1620
262 309.1694 308.1621
263 309.1694 308.1621
264 309.1695 308.1622
265 309.1695 308.1622
266 309.1695 308.1622
267 309.1695 308.1622
268 309.1695 308.1622
269 309.1696 308.1623
270 309.1696 308.1623
271 309.1696 308.1623
272 309.1696 308.1623
273 309.1698 308.1625
274 309.1698 308.1625
275 309.1698 308.1625
276 309.1698 308.1625
277 309.1701 308.1628
278 309.1875 308.1802
279 309.1881 308.1808
280 309.1882 308.1809
281 309.1882 308.1809
282 309.1884 308.1811
283 309.1886 308.1813
284 309.1889 308.1816
285 309.1891 308.1818
286 309.2062 308.1989
287 309.2062 308.1989
288 309.2063 308.1990
289 309.2063 308.1990
290 309.2065 308.1992
291 309.2067 308.1994
292 309.2070 308.1997
293 309.2072 308.1999
294 310.1115 309.1042
295 310.1115 309.1042
296 310.1116 309.1043
297 310.1707 309.1634
298 310.1707 309.1634
299 310.1913 309.1840
300 311.0565 310.0492
301 311.0728 310.0655
302 311.0807 310.0734
303 311.0814 310.0741
304 311.0908 310.0835
305 311.0913 310.0840
306 311.1104 310.1031
307 311.1110 310.1037
308 311.1112 310.1039
309 311.1113 310.1040
310 311.1115 310.1042
311 311.1117 310.1044
312 311.1118 310.1045
313 311.1121 310.1048
314 311.1472 310.1399
315 311.1479 310.1406
316 311.1482 310.1409
317 311.1482 310.1409
318 311.1483 310.1410
319 311.1483 310.1410
320 311.1484 310.1411
321 311.1485 310.1412
322 311.1486 310.1413
323 311.1487 310.1414
324 311.1490 310.1417
325 311.1490 310.1417
326 311.1490 310.1417
327 311.1492 310.1419
328 311.1492 310.1419
329 311.1493 310.1420
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