MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2
MS data file : PRT1311_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:18:13 GMT
Repeat search

[help]

Export

Not what you expected? Try the select summary.

Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,460

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

Filters

[help]

Show

Unassigned peptides, 401–500 (out of 4256)

Page: Previous 1 2 3 4 5 6 7 8 9 10 … 43 Next 

Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
130 305.1739 304.1666
131 305.1741 304.1668
132 305.1741 304.1668
133 305.1741 304.1668
134 305.1742 304.1669
135 305.1743 304.1670
136 305.1743 304.1670
137 305.1743 304.1670
138 305.1744 304.1671
139 305.1745 304.1672
140 305.1745 304.1672
141 305.1746 304.1673
142 305.1747 304.1674
143 305.1747 304.1674
144 305.1747 304.1674
145 305.1749 304.1676
146 305.1751 304.1678
147 305.1756 304.1683
148 305.1758 304.1685
149 305.1758 304.1685
150 305.1761 304.1688
151 305.1954 304.1881
152 305.1959 304.1886
153 305.2108 304.2035
154 305.2109 304.2036
155 305.2110 304.2037
156 305.2112 304.2039
157 305.9566 304.9493
158 306.0061 304.9988
159 306.0064 304.9991
160 307.0105 306.0032
161 307.0107 306.0034
162 307.0109 306.0036
163 307.0783 306.0710
164 307.0791 306.0718
165 307.1145 306.1072
166 307.1151 306.1078
167 307.1158 306.1085
168 307.1162 306.1089
169 307.1163 306.1090
170 307.1163 306.1090
171 307.1165 306.1092
172 307.1165 306.1092
173 307.1166 306.1093
174 307.1167 306.1094
175 307.1168 306.1095
176 307.1169 306.1096
177 307.1169 306.1096
178 307.1169 306.1096
179 307.1170 306.1097
180 307.1170 306.1097
181 307.1531 306.1458
182 307.1533 306.1460
183 307.1533 306.1460
184 307.1534 306.1461
185 307.1534 306.1461
186 307.1534 306.1461
187 307.1534 306.1461
188 307.1534 306.1461
189 307.1534 306.1461
190 307.1535 306.1462
191 307.1535 306.1462
192 307.1535 306.1462
193 307.1537 306.1464
194 307.1537 306.1464
195 307.1537 306.1464
196 307.1537 306.1464
197 307.1537 306.1464
198 307.1538 306.1465
199 307.1539 306.1466
200 307.1540 306.1467
201 307.1552 306.1479
202 307.1898 306.1825
203 307.1901 306.1828
204 307.1902 306.1829
205 307.1903 306.1830
206 307.1903 306.1830
207 307.1904 306.1831
208 307.1904 306.1831
209 307.1905 306.1832
210 307.1905 306.1832
211 307.1905 306.1832
212 307.1906 306.1833
213 307.1906 306.1833
214 307.1906 306.1833
215 307.1908 306.1835
216 307.1909 306.1836
217 307.1909 306.1836
218 307.1909 306.1836
219 307.1911 306.1838
220 307.1912 306.1839
221 307.2103 306.2030
222 307.2104 306.2031
223 307.2111 306.2038
224 307.9728 306.9655
225 307.9937 306.9864
226 307.9941 306.9868
227 308.1201 307.1128
228 308.1203 307.1130
229 309.0569 308.0496
Page: Previous 1 2 3 4 5 6 7 8 9 10 … 43 Next 

Not what you expected? Try the select summary.

Mascot: http://www.matrixscience.com/