MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2
MS data file : PRT1311_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:18:13 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,460

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4101–4200 (out of 4256)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4297 946.8824 2837.6254
4298 950.4662 2848.3768
4299 950.8685 2849.5837
4300 950.8693 2849.5861
4301 950.8707 2849.5903
4302 951.1964 2850.5674
4303 953.5313 2857.5721
4304 954.8080 2861.4022
4305 955.1395 2862.3967
4306 955.5831 2863.7275
4307 956.3513 2866.0321
4308 956.8186 2867.4340
4309 718.1252 2868.4717
4310 965.1829 2892.5269
4311 968.9883 2903.9431
4312 969.1397 2904.3973
4313 970.8180 2909.4322
4314 971.1500 2910.4282
4315 972.2631 2913.7675
4316 972.3696 2914.0870
4317 972.3711 2914.0915
4318 972.3752 2914.1038
4319 972.5959 2914.7659
4320 972.6011 2914.7815
4321 972.7024 2915.0854
4322 972.7065 2915.0977
4323 972.8116 2915.4130
4324 972.8163 2915.4271
4325 972.9270 2915.7592
4326 972.9318 2915.7736
4327 974.2570 2919.7492
4328 974.2625 2919.7657
4329 974.3745 2920.1017
4331 974.4803 2920.4191
4332 975.1980 2922.5722
4333 975.1984 2922.5734
4334 975.2047 2922.5923
4335 975.5374 2923.5904
4336 975.5400 2923.5982
4337 982.4733 2944.3981
4339 985.8299 2954.4679
4340 741.1417 2960.5377
4341 990.1730 2967.4972
4342 993.5030 2977.4872
4343 746.3882 2981.5237
4344 1000.4348 2998.2826
4346 1008.8483 3023.5231
4347 1008.8490 3023.5252
4348 1008.8499 3023.5279
4349 1008.8510 3023.5312
4350 1008.8513 3023.5321
4351 1009.1830 3024.5272
4352 1010.5502 3028.6288
4353 1015.8710 3044.5912
4354 1016.1764 3045.5074
4355 766.3771 3061.4793
4356 1024.5269 3070.5589
4357 1025.0225 3072.0457
4358 1026.2126 3075.6160
4359 1026.5460 3076.6162
4360 776.1979 3100.7625
4361 1034.9236 3101.7490
4362 1034.9316 3101.7730
4363 1036.5145 3106.5217
4364 1041.9210 3122.7412
4365 1048.1683 3141.4831
4366 1049.8834 3146.6284
4367 1070.8734 3209.5984
4368 1080.2151 3237.6235
4369 810.9430 3239.7429
4370 811.1923 3240.7401
4371 1086.4110 3256.2112
4372 1091.9192 3272.7358
4373 1093.9220 3278.7442
4376 820.9519 3279.7785
4377 1094.4192 3280.2358
4378 1104.5806 3310.7200
4379 1104.7012 3311.0818
4380 1115.2404 3342.6994
4381 1115.4119 3343.2139
4382 1116.5724 3346.6954
4383 679.3595 3391.7611
4384 680.1683 3395.8051
4385 850.2013 3396.7761
4386 680.3625 3396.7761
4387 850.2022 3396.7797
4388 850.2027 3396.7817
4389 1133.2690 3396.7852
4390 850.4550 3397.7909
4391 1144.2472 3429.7198
4392 1144.5901 3430.7485
4393 1144.5967 3430.7683
4394 1144.5984 3430.7734
4395 870.4564 3477.7965
4396 1160.2734 3477.7984
4397 1160.6074 3478.8004
4399 1160.6105 3478.8097
4400 870.7097 3478.8097
4401 875.9430 3499.7429
4402 1167.9276 3500.7610
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