MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2
MS data file : PRT1311_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:18:13 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,460

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4001–4100 (out of 4256)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4170 609.0953 2432.3521
4171 812.7880 2435.3422
4172 610.0647 2436.2297
4173 813.1216 2436.3430
4174 813.7823 2438.3251
4175 814.1179 2439.3319
4177 489.2534 2441.2306
4178 611.5821 2442.2993
4179 489.4680 2442.3036
4180 815.4628 2443.3666
4181 816.7300 2447.1682
4183 816.7668 2447.2786
4184 816.7697 2447.2873
4187 818.7480 2453.2222
4189 615.5762 2458.2757
4190 616.0681 2460.2433
4191 616.3519 2461.3785
4192 616.3527 2461.3817
4193 821.4694 2461.3864
4195 821.7587 2462.2543
4196 616.5712 2462.2557
4197 821.7601 2462.2585
4198 616.5723 2462.2601
4199 616.5728 2462.2621
4201 822.1061 2463.2965
4202 822.4404 2464.2994
4203 823.1208 2466.3406
4206 622.5614 2486.2165
4208 623.8244 2491.2685
4209 831.7659 2492.2759
4211 832.0891 2493.2455
4212 624.3240 2493.2669
4213 832.4220 2494.2442
4214 832.4263 2494.2571
4217 626.0574 2500.2005
4218 501.0486 2500.2066
4220 835.7716 2504.2930
4221 627.8150 2507.2309
4222 628.0733 2508.2641
4224 837.4330 2509.2772
4225 837.4415 2509.3027
4227 837.7576 2510.2510
4228 630.0682 2516.2437
4229 630.8276 2519.2813
4230 840.7678 2519.2816
4231 631.0792 2520.2877
4232 841.1041 2520.2905
4234 849.7462 2546.2168
4235 638.3312 2549.2957
4236 639.8549 2555.3905
4237 858.8850 2573.6332
4238 859.9223 2576.7451
4239 861.4421 2581.3045
4240 864.4861 2590.4365
4241 865.8071 2594.3995
4246 866.4706 2596.3900
4247 875.2426 2622.7060
4248 875.3357 2622.9853
4249 661.5793 2642.2881
4250 881.7755 2642.3047
4251 883.4716 2647.3930
4252 883.7999 2648.3779
4253 883.8001 2648.3785
4254 887.0995 2658.2767
4256 887.1021 2658.2845
4258 665.8295 2659.2889
4259 665.8300 2659.2909
4260 669.5773 2674.2801
4261 894.7854 2681.3344
4262 895.8209 2684.4409
4263 897.1695 2688.4867
4264 898.4385 2692.2937
4265 898.7755 2693.3047
4266 679.8337 2715.3057
4267 906.5406 2716.6000
4268 908.8436 2723.5090
4270 910.4557 2728.3453
4272 912.7943 2735.3611
4273 917.8345 2750.4817
4274 917.8378 2750.4916
4275 917.8391 2750.4955
4276 917.8391 2750.4955
4277 688.6315 2750.4969
4278 920.6468 2758.9186
4279 921.7943 2762.3611
4280 922.1216 2763.3430
4281 922.1259 2763.3559
4282 922.1294 2763.3664
4283 922.1350 2763.3832
4284 922.5043 2764.4911
4285 923.1650 2766.4732
4286 926.1655 2775.4747
4287 926.4958 2776.4656
4288 927.4884 2779.4434
4289 929.7886 2786.3440
4291 935.1781 2802.5125
4292 935.5172 2803.5298
4293 937.4857 2809.4353
4294 942.1225 2823.3457
4296 945.1619 2832.4639
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