MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2
MS data file : PRT1311_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:18:13 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,460

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3901–4000 (out of 4256)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4036 1093.9227 2185.8308
4037 1094.0421 2186.0696
4038 1094.1788 2186.3430
4039 1094.2985 2186.5824
4040 730.7326 2189.1760
4041 550.2516 2196.9773
4042 733.3746 2197.1020
4043 733.6655 2197.9747
4044 1104.5625 2207.1104
4046 737.7202 2210.1388
4048 738.0441 2211.1105
4049 738.0445 2211.1117
4052 740.6927 2219.0563
4053 555.7725 2219.0609
4054 741.3563 2221.0471
4056 742.7163 2225.1271
4057 1118.5400 2235.0654
4058 559.8018 2235.1781
4059 560.0503 2236.1721
4060 560.0507 2236.1737
4061 748.0393 2241.0961
4063 750.0850 2247.2332
4069 565.5729 2258.2625
4071 1134.0718 2266.1290
4072 757.0511 2268.1315
4073 568.8104 2271.2125
4074 758.4130 2272.2172
4075 759.7559 2276.2459
4076 759.7585 2276.2537
4080 571.5637 2282.2257
4081 571.5652 2282.2317
4082 457.4537 2282.2321
4084 761.7518 2282.2336
4085 457.4540 2282.2336
4087 571.5659 2282.2345
4091 571.5662 2282.2357
4093 571.5666 2282.2373
4096 762.7409 2285.2009
4097 572.5572 2286.1997
4098 763.4308 2287.2706
4099 765.0814 2292.2224
4100 765.4138 2293.2196
4101 765.4179 2293.2319
4102 765.4182 2293.2328
4103 765.4186 2293.2340
4105 767.7301 2300.1685
4106 1152.0586 2302.1026
4107 1152.0592 2302.1038
4108 768.3764 2302.1074
4109 768.7490 2303.2252
4110 1152.6205 2303.2264
4111 768.7499 2303.2279
4112 1152.6281 2303.2416
4114 769.0994 2304.2764
4116 771.0829 2310.2269
4119 776.4038 2326.1896
4120 776.4044 2326.1914
4122 585.8213 2339.2561
4123 781.0637 2340.1693
4124 781.0748 2340.2026
4125 781.0776 2340.2110
4127 781.4055 2341.1947
4128 586.3065 2341.1969
4129 586.3097 2341.2097
4131 1172.6238 2343.2330
4132 784.7198 2351.1376
4133 785.0654 2352.1744
4134 788.3884 2362.1434
4135 593.8058 2371.1941
4136 593.8216 2371.2573
4137 1188.1561 2374.2976
4138 795.9422 2384.8048
4139 795.9434 2384.8084
4140 597.5933 2386.3441
4141 796.4555 2386.3447
4142 597.5941 2386.3473
4143 797.0936 2388.2590
4144 797.7626 2390.2660
4145 598.8128 2391.2221
4146 798.4001 2392.1785
4147 799.1146 2394.3220
4148 800.7688 2399.2846
4149 800.7863 2399.3371
4151 801.7578 2402.2516
4153 602.0951 2404.3513
4154 802.4584 2404.3534
4155 802.4588 2404.3546
4156 602.0962 2404.3557
4157 602.0963 2404.3561
4159 802.4602 2404.3588
4160 804.7893 2411.3461
4161 806.1003 2415.2791
4162 806.4402 2416.2988
4163 807.4539 2419.3399
4164 807.4542 2419.3408
4165 486.6702 2428.3146
4166 608.0860 2428.3149
4167 608.8441 2431.3473
4168 811.7872 2432.3398
4169 811.7908 2432.3506
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