MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2
MS data file : PRT1311_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:18:13 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,460

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 301–400 (out of 4256)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
30 301.1425 300.1352
31 301.1425 300.1352
32 301.1425 300.1352
33 301.1425 300.1352
34 301.1425 300.1352
35 301.1425 300.1352
36 301.1425 300.1352
37 301.1425 300.1352
38 301.1425 300.1352
39 301.1425 300.1352
40 301.1426 300.1353
41 301.1426 300.1353
42 301.1426 300.1353
43 301.1427 300.1354
44 301.1428 300.1355
45 301.1428 300.1355
46 301.1428 300.1355
47 301.1428 300.1355
48 301.1428 300.1355
49 301.1429 300.1356
50 301.1429 300.1356
51 301.1429 300.1356
52 301.1432 300.1359
53 301.1432 300.1359
54 301.2212 300.2139
55 301.2215 300.2142
56 301.2216 300.2143
57 302.1979 301.1906
58 303.0671 302.0598
59 303.0835 302.0762
60 303.0836 302.0763
61 303.0838 302.0765
62 303.0838 302.0765
63 303.0842 302.0769
64 303.0848 302.0775
65 303.0851 302.0778
66 303.0855 302.0782
67 303.1024 302.0951
68 303.1211 302.1138
69 303.1214 302.1141
70 303.1214 302.1141
71 303.1215 302.1142
72 303.1216 302.1143
73 303.1216 302.1143
74 303.1217 302.1144
75 303.1218 302.1145
76 303.1218 302.1145
77 303.1218 302.1145
78 303.1218 302.1145
79 303.1218 302.1145
80 303.1219 302.1146
81 303.1219 302.1146
82 303.1219 302.1146
83 303.1220 302.1147
84 303.1223 302.1150
85 303.1226 302.1153
86 303.1792 302.1719
87 303.1793 302.1720
88 303.1793 302.1720
89 304.9722 303.9649
90 304.9726 303.9653
91 304.9727 303.9654
92 305.0983 304.0910
93 305.0984 304.0911
94 305.0984 304.0911
95 305.0990 304.0917
96 305.0999 304.0926
97 305.1004 304.0931
98 305.1005 304.0932
99 305.1008 304.0935
100 305.1008 304.0935
101 305.1009 304.0936
102 305.1013 304.0940
103 305.1013 304.0940
104 305.1175 304.1102
105 305.1370 304.1297
106 305.1371 304.1298
107 305.1372 304.1299
108 305.1375 304.1302
109 305.1375 304.1302
110 305.1375 304.1302
111 305.1375 304.1302
112 305.1376 304.1303
113 305.1376 304.1303
114 305.1376 304.1303
115 305.1376 304.1303
116 305.1377 304.1304
117 305.1377 304.1304
118 305.1377 304.1304
119 305.1377 304.1304
120 305.1378 304.1305
121 305.1378 304.1305
122 305.1378 304.1305
123 305.1378 304.1305
124 305.1379 304.1306
125 305.1380 304.1307
126 305.1380 304.1307
127 305.1381 304.1308
128 305.1588 304.1515
129 305.1738 304.1665
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