MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2
MS data file : PRT1311_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:18:13 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,460

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3801–3900 (out of 4256)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3891 603.3214 1806.9424
3894 904.4824 1806.9502
3895 603.9938 1808.9596
3896 906.7425 1811.4704
3897 606.9898 1817.9476
3898 606.9922 1817.9548
3900 912.0067 1821.9988
3901 912.4543 1822.8940
3903 912.5104 1823.0062
3909 922.1500 1842.2854
3910 461.9797 1843.8897
3911 615.9717 1844.8933
3913 616.3143 1845.9211
3914 924.2711 1846.5276
3918 621.3161 1860.9265
3920 932.9911 1863.9676
3921 622.6260 1864.8562
3922 623.0361 1866.0865
3923 934.0511 1866.0876
3924 623.3686 1867.0840
3925 934.5494 1867.0842
3927 627.6750 1880.0032
3928 941.4797 1880.9448
3929 628.0094 1881.0064
3930 629.2838 1884.8296
3933 634.0195 1899.0367
3934 955.7744 1909.5342
3935 958.1870 1914.3594
3936 958.5259 1915.0372
3937 480.0167 1916.0377
3939 967.0117 1932.0088
3940 645.3415 1933.0027
3941 645.3437 1933.0093
3942 645.3438 1933.0096
3943 645.3441 1933.0105
3945 973.0392 1944.0638
3946 974.3701 1946.7256
3947 974.4853 1946.9560
3950 652.3351 1953.9835
3953 653.3446 1957.0120
3954 1002.0305 2002.0464
3955 670.3653 2008.0741
3956 1006.6358 2011.2570
3957 1010.3544 2018.6942
3958 1012.5192 2023.0238
3959 1012.5224 2023.0302
3960 676.6880 2027.0422
3961 1016.5197 2031.0248
3962 1016.7715 2031.5284
3963 1016.7721 2031.5296
3964 1017.0262 2032.0378
3965 1017.2819 2032.5492
3967 686.0265 2055.0577
3968 1030.0220 2058.0294
3971 1030.0297 2058.0448
3973 688.0612 2061.1618
3979 695.3774 2083.1104
3980 1042.5701 2083.1256
3982 1043.8020 2085.5894
3983 699.3752 2095.1038
3984 706.0579 2115.1519
3985 713.0684 2136.1834
3986 713.4019 2137.1839
3987 713.4031 2137.1875
3988 713.4031 2137.1875
3989 713.4039 2137.1899
3990 713.4039 2137.1899
3991 1070.5378 2139.0610
3993 717.3593 2149.0561
3995 1079.5560 2157.0974
3996 720.0699 2157.1879
3998 720.0707 2157.1903
3999 540.3051 2157.1913
4005 1080.5197 2159.0248
4007 1080.5214 2159.0282
4008 434.8303 2169.1151
4009 543.2871 2169.1193
4010 724.7296 2171.1670
4011 725.0339 2172.0799
4012 726.4218 2176.2436
4013 726.4221 2176.2445
4014 545.0684 2176.2445
4015 545.0685 2176.2449
4016 727.3933 2179.1581
4017 1091.5487 2181.0828
4018 728.0417 2181.1033
4019 728.0421 2181.1045
4020 1091.5599 2181.1052
4021 1091.5602 2181.1058
4022 1091.5603 2181.1060
4023 728.0428 2181.1066
4024 1091.5610 2181.1074
4025 728.0431 2181.1075
4028 728.0446 2181.1120
4029 728.0453 2181.1141
4030 1091.5645 2181.1144
4032 1092.0583 2182.1020
4033 728.3783 2182.1131
4034 1092.5477 2183.0808
4035 1093.9171 2185.8196
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