MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2
MS data file : PRT1311_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:18:13 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,460

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3701–3800 (out of 4256)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3709 691.8319 1381.6492
3710 691.8774 1381.7402
3711 693.3564 1384.6982
3712 693.3573 1384.7000
3714 693.3588 1384.7030
3718 463.5733 1387.6981
3721 469.9307 1406.7703
3722 704.3934 1406.7722
3723 470.2656 1407.7750
3728 471.9722 1412.8948
3732 712.8625 1423.7104
3733 713.8728 1425.7310
3734 477.6322 1429.8748
3735 716.3875 1430.7604
3744 478.3105 1431.9097
3745 717.4271 1432.8396
3747 720.3160 1438.6174
3748 721.8694 1441.7242
3751 730.3798 1458.7450
3752 487.3078 1458.9016
3753 735.4017 1468.7888
3754 368.9900 1471.9309
3755 370.1989 1476.7665
3762 739.9310 1477.8474
3763 493.9570 1478.8492
3764 372.9887 1487.9257
3765 372.9892 1487.9277
3766 372.9893 1487.9281
3767 496.9835 1487.9287
3768 496.9836 1487.9290
3769 746.4371 1490.8596
3770 498.2820 1491.8242
3778 377.4676 1505.8413
3780 504.5988 1510.7746
3783 512.6303 1534.8691
3791 518.6132 1552.8178
3794 784.4482 1566.8818
3797 786.9274 1571.8402
3798 527.6164 1579.8274
3799 791.9452 1581.8758
3800 528.2998 1581.8776
3801 796.1243 1590.2340
3803 534.9634 1601.8684
3804 810.4406 1618.8666
3805 541.9781 1622.9125
3807 547.9611 1640.8615
3808 826.4279 1650.8412
3809 414.7295 1654.8889
3811 552.6374 1654.8904
3812 835.9440 1669.8734
3813 837.1000 1672.1854
3815 558.6398 1672.8976
3817 419.2317 1672.8977
3818 419.2319 1672.8985
3819 419.2319 1672.8985
3820 558.6405 1672.8997
3821 558.6407 1672.9003
3823 419.4829 1673.9025
3824 841.9709 1681.9272
3826 847.8837 1693.7528
3828 849.3649 1696.7152
3829 425.2191 1696.8473
3830 849.9025 1697.7904
3833 567.9728 1700.8966
3835 853.9665 1705.9184
3836 854.3761 1706.7376
3838 571.2681 1710.7825
3839 428.7184 1710.8445
3840 571.9790 1712.9152
3841 571.9794 1712.9164
3842 857.9585 1713.9024
3843 860.4245 1718.8344
3846 863.4767 1724.9388
3847 575.9870 1724.9392
3849 575.9874 1724.9404
3850 575.9875 1724.9407
3853 866.3433 1730.6720
3855 433.7385 1730.9249
3859 582.6602 1744.9588
3860 873.9294 1745.8442
3861 583.6392 1747.8958
3862 875.2337 1748.4528
3863 875.3373 1748.6600
3864 875.5317 1749.0488
3865 875.5359 1749.0572
3866 876.1956 1750.3766
3867 584.9835 1751.9287
3872 588.3010 1761.8812
3874 885.4502 1768.8858
3876 886.9442 1771.8738
3877 886.9457 1771.8768
3878 887.4486 1772.8826
3879 594.6602 1780.9588
3880 594.9948 1781.9626
3881 896.9494 1791.8842
3882 598.3174 1791.9304
3883 599.6772 1796.0098
3884 600.3446 1798.0120
3885 600.3453 1798.0141
3886 601.3297 1800.9673
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