MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2
MS data file : PRT1311_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:18:13 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,460

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3601–3700 (out of 4256)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3530 374.5402 1120.5988
3531 562.8419 1123.6692
3533 377.5484 1129.6234
3534 565.8199 1129.6252
3537 379.2119 1134.6139
3538 570.8448 1139.6750
3539 1144.2626 1143.2553
3540 575.3182 1148.6218
3541 575.3189 1148.6232
3542 575.3560 1148.6974
3543 575.3565 1148.6984
3544 575.3566 1148.6986
3545 575.3571 1148.6996
3547 386.1876 1155.5410
3548 579.3214 1156.6282
3549 389.5493 1165.6261
3553 390.8706 1169.5900
3557 589.8155 1177.6164
3559 396.5456 1186.6150
3564 396.8839 1187.6299
3565 396.8841 1187.6305
3566 594.8448 1187.6750
3567 397.2234 1188.6484
3568 397.5946 1189.7620
3569 595.8889 1189.7632
3570 397.9267 1190.7583
3571 597.8187 1193.6228
3572 600.8384 1199.6622
3576 303.3968 1209.5581
3577 404.1934 1209.5584
3579 404.1937 1209.5593
3580 303.3972 1209.5597
3581 404.5893 1210.7461
3583 404.5894 1210.7464
3584 404.5896 1210.7470
3586 606.3812 1210.7478
3587 606.3815 1210.7484
3588 607.8461 1213.6776
3591 608.3397 1214.6648
3594 307.8995 1227.5689
3595 614.7921 1227.5696
3596 410.1973 1227.5701
3598 410.1974 1227.5704
3599 614.7925 1227.5704
3600 410.1977 1227.5713
3608 411.9110 1232.7112
3609 411.9112 1232.7118
3610 411.9116 1232.7130
3612 618.3010 1234.5874
3618 310.4221 1237.6593
3619 413.5606 1237.6600
3621 620.3787 1238.7428
3622 620.8701 1239.7256
3625 622.8195 1243.6244
3628 416.2627 1245.7663
3629 417.2388 1248.6946
3630 418.1836 1251.5290
3633 419.5291 1255.5655
3637 630.8483 1259.6820
3638 630.8488 1259.6830
3639 630.8503 1259.6860
3640 630.8505 1259.6864
3644 421.2733 1260.7981
3646 632.3627 1262.7108
3647 633.3253 1264.6360
3649 423.2007 1266.5803
3650 423.5347 1267.5823
3651 423.5972 1267.7698
3652 318.4162 1269.6357
3655 424.2197 1269.6373
3656 636.8167 1271.6188
3662 429.2041 1284.5905
3663 429.2042 1284.5908
3664 643.8105 1285.6064
3665 429.5428 1285.6066
3666 645.3627 1288.7108
3667 645.3630 1288.7114
3668 430.5812 1288.7218
3670 431.1936 1290.5590
3675 647.8694 1293.7242
3678 435.2017 1302.5833
3679 436.5576 1306.6510
3680 658.8734 1315.7322
3681 659.3599 1316.7052
3682 659.9177 1317.8208
3683 440.2812 1317.8218
3684 440.2812 1317.8218
3685 665.3707 1328.7268
3686 444.9199 1331.7379
3687 675.3640 1348.7134
3697 451.1672 1350.4798
3699 680.8928 1359.7710
3700 454.2648 1359.7726
3701 682.8717 1363.7288
3702 685.8687 1369.7228
3703 686.3671 1370.7196
3704 458.9100 1373.7082
3706 689.8782 1377.7418
3707 460.2550 1377.7432
3708 689.8796 1377.7446
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