MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2
MS data file : PRT1311_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:18:13 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,460

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3501–3600 (out of 4256)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3326 435.7767 869.5388
3327 435.7769 869.5392
3328 436.7844 871.5542
3330 874.3292 873.3219
3331 874.4475 873.4402
3332 875.4382 874.4309
3333 875.6331 874.6258
3335 885.1160 884.1087
3336 885.6355 884.6282
3337 887.9476 886.9403
3338 889.2323 888.2250
3339 889.4411 888.4338
3341 898.4406 897.4333
3342 449.7736 897.5326
3344 452.0852 902.1558
3347 919.7812 918.7739
3349 922.5767 921.5694
3350 922.5773 921.5700
3351 461.7924 921.5702
3352 461.7928 921.5710
3353 928.2127 927.2054
3354 464.7432 927.4718
3356 467.1936 932.3726
3357 934.0466 933.0393
3358 468.7152 935.4158
3359 937.6117 936.6044
3360 314.1714 939.4924
3361 470.7543 939.4940
3368 947.4533 946.4460
3370 476.7668 951.5190
3371 955.1385 954.1312
3372 955.4682 954.4609
3375 956.2062 955.1989
3376 956.4809 955.4736
3377 956.8225 955.8152
3378 481.3159 960.6172
3379 969.4880 968.4807
3381 969.5994 968.5921
3382 970.4813 969.4740
3383 970.7020 969.6947
3384 324.4880 970.4422
3385 486.7164 971.4182
3386 972.4833 971.4760
3387 972.5966 971.5893
3388 973.4940 972.4867
3389 487.2730 972.5314
3390 973.6052 972.5979
3391 974.2522 973.2449
3397 974.6473 973.6400
3404 490.8183 979.6220
3405 981.4604 980.4531
3410 328.8170 983.4292
3413 989.5705 988.5632
3414 330.8401 989.4985
3416 496.2483 990.4820
3417 496.2484 990.4822
3418 496.2491 990.4836
3420 496.8069 991.5992
3425 500.7068 999.3990
3429 335.8111 1004.4115
3430 503.2140 1004.4134
3434 1009.5255 1008.5182
3435 506.2409 1010.4672
3438 507.2227 1012.4308
3441 1016.5292 1015.5219
3442 1018.0161 1017.0088
3443 340.4748 1018.4026
3445 513.7935 1025.5724
3453 516.7611 1031.5076
3455 519.2777 1036.5408
3456 346.8632 1037.5678
3458 346.9004 1037.6794
3459 346.9006 1037.6800
3460 346.9008 1037.6806
3461 346.9009 1037.6809
3466 523.2928 1044.5710
3470 528.3014 1054.5882
3477 531.2224 1060.4302
3482 355.8523 1064.5351
3483 537.2872 1072.5598
3484 1074.5333 1073.5260
3485 537.8401 1073.6656
3497 1086.1578 1085.1505
3498 544.2667 1086.5188
3499 363.2010 1086.5812
3500 363.2171 1086.6295
3502 1091.5590 1090.5517
3503 1091.5648 1090.5575
3504 1091.6675 1090.6602
3510 546.8459 1091.6772
3512 1094.5381 1093.5308
3513 548.3016 1094.5886
3516 554.3116 1106.6086
3519 370.8846 1109.6320
3520 558.7792 1115.5438
3521 558.8244 1115.6342
3526 560.8433 1119.6720
3527 560.8433 1119.6720
3528 560.8441 1119.6736
3529 374.5400 1120.5982
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