MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2
MS data file : PRT1311_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:18:13 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,460

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3401–3500 (out of 4256)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3156 621.3760 620.3687
3158 625.4073 624.4000
3159 313.5374 625.0602
3160 626.0771 625.0698
3161 314.7067 627.3988
3167 318.1241 634.2336
3168 636.0322 635.0249
3169 636.9376 635.9303
3170 319.0772 636.1398
3171 319.1547 636.2948
3176 641.3768 640.3695
3185 642.4339 641.4266
3186 642.4345 641.4272
3187 646.3345 645.3272
3188 325.1282 648.2418
3189 325.1638 648.3130
3190 325.1646 648.3146
3191 649.3707 648.3634
3194 325.6685 649.3224
3195 651.2122 650.2049
3196 652.3448 651.3375
3197 652.9106 651.9033
3198 327.1219 652.2292
3199 327.1446 652.2746
3200 327.1446 652.2746
3201 657.3017 656.2944
3203 330.0492 658.0838
3205 332.0839 662.1532
3206 664.3435 663.3362
3208 665.3284 664.3211
3209 335.1487 668.2828
3210 670.4276 669.4203
3211 670.4289 669.4216
3212 672.4075 671.4002
3213 672.4080 671.4007
3214 339.7026 677.3906
3215 340.0284 678.0422
3216 340.0515 678.0884
3217 340.7044 679.3942
3218 680.8911 679.8838
3222 696.4443 695.4370
3223 348.7259 695.4372
3224 697.4285 696.4212
3227 702.4544 701.4471
3228 702.4553 701.4480
3229 702.9790 701.9717
3231 355.1392 708.2638
3232 712.4136 711.4063
3233 718.9524 717.9451
3236 361.0584 720.1022
3237 722.4600 721.4527
3238 723.3505 722.3432
3239 723.3824 722.3751
3240 725.4589 724.4516
3241 725.4601 724.4528
3242 364.1767 726.3388
3245 369.1549 736.2952
3246 738.8712 737.8639
3249 372.5337 743.0528
3250 744.9904 743.9831
3251 744.9907 743.9834
3253 755.4689 754.4616
3255 760.9619 759.9546
3256 760.9633 759.9560
3257 763.9448 762.9375
3258 765.4161 764.4088
3259 383.7160 765.4174
3260 770.3157 769.3084
3261 770.3878 769.3805
3262 385.7013 769.3880
3269 787.0003 785.9930
3270 796.0335 795.0262
3271 796.1259 795.1186
3272 399.2678 796.5210
3273 802.9717 801.9644
3276 404.2088 806.4030
3277 404.7448 807.4750
3278 809.4901 808.4828
3279 809.4922 808.4849
3280 405.7700 809.5254
3285 815.5394 814.5321
3287 815.5396 814.5323
3291 817.4358 816.4285
3292 409.2747 816.5348
3294 823.4562 822.4489
3295 412.6900 823.3654
3296 412.7555 823.4964
3298 413.2195 824.4244
3300 832.7546 831.7473
3301 420.2527 838.4908
3302 420.7402 839.4658
3304 842.5111 841.5038
3311 422.2714 842.5282
3312 843.5360 842.5287
3314 423.7216 845.4286
3315 851.4008 850.3935
3316 427.2474 852.4802
3319 859.3469 858.3396
3321 865.7946 864.7873
3322 434.2759 866.5372
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