MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2
MS data file : PRT1311_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:18:13 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,460

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3301–3400 (out of 4256)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3031 502.3372 501.3299
3032 502.3378 501.3305
3033 504.2454 503.2381
3034 504.8364 503.8291
3035 504.8365 503.8292
3036 504.8366 503.8293
3037 504.8369 503.8296
3038 504.8369 503.8296
3039 504.8372 503.8299
3040 507.1075 506.1002
3041 508.1090 507.1017
3042 508.1703 507.1630
3043 508.2909 507.2836
3044 508.2914 507.2841
3045 508.2918 507.2845
3046 508.2920 507.2847
3047 509.2000 508.1927
3048 510.1241 509.1168
3049 511.0523 510.0450
3050 511.1792 510.1719
3051 511.1799 510.1726
3052 512.3226 511.3153
3053 513.1948 512.1875
3054 513.1949 512.1876
3057 516.3179 515.3106
3058 516.3181 515.3108
3059 518.1118 517.1045
3060 522.1248 521.1175
3061 522.1249 521.1176
3062 525.1949 524.1876
3063 527.2096 526.2023
3064 527.3333 526.3260
3067 529.1903 528.1830
3068 529.3475 528.3402
3069 529.3484 528.3411
3071 529.3494 528.3421
3072 531.3074 530.3001
3073 532.1285 531.1212
3074 532.1289 531.1216
3075 533.1060 532.0987
3076 534.9189 533.9116
3077 538.0868 537.0795
3078 538.1195 537.1122
3079 538.9537 537.9464
3080 539.1334 538.1261
3081 539.2103 538.2030
3082 541.1898 540.1825
3083 543.3282 542.3209
3085 545.0023 543.9950
3087 547.9625 546.9552
3088 552.1351 551.1278
3089 552.1353 551.1280
3090 553.1491 552.1418
3091 554.9257 553.9184
3092 555.0314 554.0241
3093 555.2055 554.1982
3094 555.2062 554.1989
3095 561.0776 560.0703
3096 562.0440 561.0367
3097 562.0594 561.0521
3098 562.0887 561.0814
3099 566.1180 565.1107
3100 566.1194 565.1121
3101 567.1283 566.1210
3102 567.2446 566.2373
3103 568.3065 567.2992
3104 569.2211 568.2138
3106 574.3466 573.3393
3107 576.0504 575.0431
3108 582.0511 581.0438
3109 582.1135 581.1062
3110 583.8203 582.8130
3111 586.0200 585.0127
3112 586.9207 585.9134
3113 587.3549 586.3476
3117 589.3700 588.3627
3118 589.3702 588.3629
3119 589.3703 588.3630
3121 592.0057 590.9984
3122 594.4014 593.3941
3123 598.3038 597.2965
3126 602.8930 601.8857
3127 603.0883 602.0810
3128 302.5365 603.0584
3129 606.0701 605.0628
3130 606.9939 605.9866
3133 306.1414 610.2682
3134 612.3752 611.3679
3135 614.2603 613.2530
3136 307.6339 613.2532
3138 309.0419 616.0692
3139 309.0428 616.0710
3140 309.0945 616.1744
3141 309.1692 616.3238
3142 617.3656 616.3583
3143 618.0611 617.0538
3149 310.5333 619.0520
3150 311.1105 620.2064
3151 621.2526 620.2453
3154 621.3755 620.3682
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