MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2
MS data file : PRT1311_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:18:13 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,460

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3201–3300 (out of 4256)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2930 455.2704 454.2631
2931 455.2705 454.2632
2932 455.2705 454.2632
2933 455.2705 454.2632
2934 455.2705 454.2632
2935 455.2706 454.2633
2936 455.2708 454.2635
2937 455.2708 454.2635
2938 455.2708 454.2635
2939 455.2710 454.2637
2940 455.2711 454.2638
2941 455.2712 454.2639
2942 457.1653 456.1580
2943 457.1680 456.1607
2944 461.2556 460.2483
2945 461.2560 460.2487
2946 461.2569 460.2496
2947 462.0872 461.0799
2948 462.0874 461.0801
2949 462.2338 461.2265
2950 462.2339 461.2266
2951 463.2322 462.2249
2952 463.2332 462.2259
2953 463.2341 462.2268
2954 463.2499 462.2426
2955 464.1020 463.0947
2956 465.0257 464.0184
2957 465.0961 464.0888
2958 465.2114 464.2041
2959 465.2127 464.2054
2960 465.2127 464.2054
2961 465.2129 464.2056
2962 465.2129 464.2056
2963 465.2130 464.2057
2964 465.2131 464.2058
2965 465.2132 464.2059
2966 465.2479 464.2406
2967 466.0981 465.0908
2968 467.1519 466.1446
2969 467.1882 466.1809
2970 467.2278 466.2205
2971 467.2281 466.2208
2972 467.2281 466.2208
2973 467.2282 466.2209
2974 467.2284 466.2211
2975 467.2288 466.2215
2976 468.1131 467.1058
2977 469.1677 468.1604
2978 469.1681 468.1608
2979 469.2437 468.2364
2980 469.2439 468.2366
2981 470.7542 469.7469
2982 471.1356 470.1283
2983 471.1820 470.1747
2984 471.1835 470.1762
2985 472.2905 471.2832
2986 473.0516 472.0443
2987 473.1497 472.1424
2988 473.1508 472.1435
2989 473.1614 472.1541
2990 474.9798 473.9725
2991 474.9798 473.9725
2992 476.1015 475.0942
2993 476.2851 475.2778
2994 476.2856 475.2783
2995 476.2856 475.2783
2996 476.2856 475.2783
2997 476.9346 475.9273
2998 477.2118 476.2045
2999 479.2278 478.2205
3000 479.2293 478.2220
3001 479.2650 478.2577
3002 480.9839 479.9766
3003 481.1685 480.1612
3004 481.2072 480.1999
3005 481.2076 480.2003
3006 483.0581 482.0508
3007 483.1829 482.1756
3008 483.1833 482.1760
3009 485.1628 484.1555
3010 485.7273 484.7200
3012 489.2444 488.2371
3013 490.0915 489.0842
3014 492.0967 491.0894
3015 492.9063 491.8990
3016 492.9073 491.9000
3017 492.9076 491.9003
3018 493.1681 492.1608
3019 493.2443 492.2370
3020 495.0358 494.0285
3021 495.1837 494.1764
3022 496.1085 495.1012
3023 496.2899 495.2826
3024 497.1638 496.1565
3025 497.1698 496.1625
3026 497.1992 496.1919
3027 499.1672 498.1599
3028 499.1781 498.1708
3029 499.1786 498.1713
3030 499.1787 498.1714
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