MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2
MS data file : PRT1311_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:18:13 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,460

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3101–3200 (out of 4256)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2830 439.2150 438.2077
2831 439.2151 438.2078
2832 439.2152 438.2079
2833 439.2154 438.2081
2834 439.2162 438.2089
2835 439.2325 438.2252
2836 439.2326 438.2253
2837 439.2326 438.2253
2838 439.2328 438.2255
2839 439.2329 438.2256
2840 439.2331 438.2258
2841 439.2332 438.2259
2842 440.9746 439.9673
2843 440.9755 439.9682
2844 441.1721 440.1648
2845 441.1729 440.1656
2846 441.2108 440.2035
2847 441.2305 440.2232
2848 441.2306 440.2233
2849 441.2306 440.2233
2850 441.2306 440.2233
2851 441.2308 440.2235
2852 441.2311 440.2238
2853 441.2319 440.2246
2854 443.0432 442.0359
2855 443.1500 442.1427
2856 443.2463 442.2390
2857 443.2464 442.2391
2858 443.2464 442.2391
2859 443.2466 442.2393
2860 443.2468 442.2395
2861 443.2468 442.2395
2862 443.2469 442.2396
2863 443.2473 442.2400
2864 445.0188 444.0115
2865 445.0194 444.0121
2866 445.0205 444.0132
2867 446.2755 445.2682
2868 447.2021 446.1948
2869 447.2784 446.2711
2870 449.1518 448.1445
2871 449.1524 448.1451
2872 449.1785 448.1712
2873 449.1808 448.1735
2874 449.1810 448.1737
2875 449.1815 448.1742
2876 449.1817 448.1744
2877 449.2168 448.2095
2878 449.2171 448.2098
2879 449.2176 448.2103
2880 449.2177 448.2104
2881 449.2177 448.2104
2882 449.2182 448.2109
2883 450.8956 449.8883
2884 450.8962 449.8889
2885 451.1572 450.1499
2886 451.1679 450.1606
2887 451.1688 450.1615
2888 451.1952 450.1879
2889 451.1964 450.1891
2890 451.1964 450.1891
2891 451.1966 450.1893
2892 451.1967 450.1894
2893 451.1969 450.1896
2894 451.1970 450.1897
2895 451.1970 450.1897
2896 451.1970 450.1897
2897 451.1971 450.1898
2898 451.1973 450.1900
2899 451.1991 450.1918
2900 451.2333 450.2260
2901 451.2334 450.2261
2902 451.2340 450.2267
2903 451.2342 450.2269
2904 451.2346 450.2273
2905 452.8965 451.8892
2906 453.1728 452.1655
2907 453.1739 452.1666
2908 453.1922 452.1849
2909 453.2111 452.2038
2910 453.2114 452.2041
2911 453.2119 452.2046
2912 453.2122 452.2049
2913 453.2122 452.2049
2914 453.2124 452.2051
2915 453.2125 452.2052
2916 453.2125 452.2052
2917 453.2126 452.2053
2918 453.2126 452.2053
2919 453.2127 452.2054
2920 453.2128 452.2055
2921 453.2129 452.2056
2922 453.2133 452.2060
2923 453.3460 452.3387
2924 455.1498 454.1425
2925 455.1516 454.1443
2926 455.1786 454.1713
2927 455.1884 454.1811
2928 455.2281 454.2208
2929 455.2704 454.2631
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