MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2
MS data file : PRT1311_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:18:13 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,460

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3001–3100 (out of 4256)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2730 427.2693 426.2620
2731 427.2695 426.2622
2732 427.2700 426.2627
2733 427.2715 426.2642
2734 427.3192 426.3119
2735 427.3195 426.3122
2736 429.0905 428.0832
2737 429.0906 428.0833
2738 429.0908 428.0835
2739 429.0912 428.0839
2740 429.0912 428.0839
2741 429.0912 428.0839
2742 429.0914 428.0841
2743 429.0914 428.0841
2744 429.0915 428.0842
2745 429.0917 428.0844
2746 429.0920 428.0847
2747 429.1544 428.1471
2748 429.2679 428.2606
2749 429.2683 428.2610
2750 429.2844 428.2771
2751 430.2796 429.2723
2752 431.2389 430.2316
2753 431.2394 430.2321
2754 431.2395 430.2322
2755 432.2212 431.2139
2756 432.8861 431.8788
2757 433.1857 432.1784
2758 433.1858 432.1785
2759 433.1859 432.1786
2760 433.1862 432.1789
2761 433.1863 432.1790
2762 433.1867 432.1794
2763 434.9231 433.9158
2764 434.9240 433.9167
2765 435.0741 434.0668
2766 435.1652 434.1579
2767 435.2013 434.1940
2768 435.2015 434.1942
2769 435.2016 434.1943
2770 435.2017 434.1944
2771 435.2018 434.1945
2772 435.2018 434.1945
2773 435.2018 434.1945
2774 435.2019 434.1946
2775 435.2020 434.1947
2776 435.2021 434.1948
2777 435.2021 434.1948
2778 435.2022 434.1949
2779 435.2022 434.1949
2780 435.2023 434.1950
2781 435.2383 434.2310
2782 437.1793 436.1720
2783 437.1798 436.1725
2784 437.1809 436.1736
2785 437.1809 436.1736
2786 437.1811 436.1738
2787 437.1811 436.1738
2788 437.1812 436.1739
2789 437.1813 436.1740
2790 437.1815 436.1742
2791 437.1955 436.1882
2792 437.1981 436.1908
2793 437.2160 436.2087
2794 437.2167 436.2094
2795 437.2172 436.2099
2796 437.2172 436.2099
2797 437.2173 436.2100
2798 437.2174 436.2101
2799 437.2174 436.2101
2800 437.2174 436.2101
2801 437.2174 436.2101
2802 437.2174 436.2101
2803 437.2174 436.2101
2804 437.2175 436.2102
2805 437.2175 436.2102
2806 437.2178 436.2105
2807 437.2178 436.2105
2808 437.2179 436.2106
2809 437.2180 436.2107
2810 437.2383 436.2310
2811 438.1028 437.0955
2812 439.0714 438.0641
2813 439.1572 438.1499
2814 439.1573 438.1500
2815 439.1951 438.1878
2816 439.1964 438.1891
2817 439.1964 438.1891
2818 439.1968 438.1895
2819 439.1969 438.1896
2820 439.1970 438.1897
2821 439.1971 438.1898
2822 439.1972 438.1899
2823 439.2144 438.2071
2824 439.2147 438.2074
2825 439.2147 438.2074
2826 439.2149 438.2076
2827 439.2149 438.2076
2828 439.2149 438.2076
2829 439.2149 438.2076
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