MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2
MS data file : PRT1311_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:18:13 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,460

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2901–3000 (out of 4256)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2630 421.2228 420.2155
2631 421.2229 420.2156
2632 421.2229 420.2156
2633 423.1625 422.1552
2634 423.1635 422.1562
2635 423.1645 422.1572
2636 423.1651 422.1578
2637 423.1653 422.1580
2638 423.1655 422.1582
2639 423.1655 422.1582
2640 423.1656 422.1583
2641 423.1991 422.1918
2642 423.1993 422.1920
2643 423.1993 422.1920
2644 423.1996 422.1923
2645 423.1996 422.1923
2646 423.1999 422.1926
2647 423.2002 422.1929
2648 423.2004 422.1931
2649 423.2005 422.1932
2650 423.2013 422.1940
2651 423.2013 422.1940
2652 423.2015 422.1942
2653 423.2015 422.1942
2654 423.2015 422.1942
2655 423.2015 422.1942
2656 423.2016 422.1943
2657 423.2016 422.1943
2658 423.2018 422.1945
2659 423.2018 422.1945
2660 423.2018 422.1945
2661 423.2018 422.1945
2662 423.2018 422.1945
2663 423.2019 422.1946
2664 423.2021 422.1948
2665 423.2023 422.1950
2666 423.2196 422.2123
2667 423.2198 422.2125
2668 423.2198 422.2125
2669 423.2198 422.2125
2670 423.2198 422.2125
2671 423.2199 422.2126
2672 423.2199 422.2126
2673 423.2199 422.2126
2674 423.2201 422.2128
2675 423.2201 422.2128
2676 423.2204 422.2131
2677 423.2212 422.2139
2678 423.2215 422.2142
2679 423.2925 422.2852
2680 424.0694 423.0621
2681 424.9981 423.9908
2682 425.1407 424.1334
2683 425.1418 424.1345
2684 425.1506 424.1433
2685 425.1798 424.1725
2686 425.1802 424.1729
2687 425.1804 424.1731
2688 425.1805 424.1732
2689 425.1808 424.1735
2690 425.1809 424.1736
2691 425.1812 424.1739
2692 425.1814 424.1741
2693 425.1814 424.1741
2694 425.2168 424.2095
2695 425.2175 424.2102
2696 425.2177 424.2104
2697 425.2178 424.2105
2698 425.2178 424.2105
2699 425.2353 424.2280
2700 425.2353 424.2280
2701 425.2353 424.2280
2702 425.2354 424.2281
2703 425.2355 424.2282
2704 425.2357 424.2284
2705 425.2358 424.2285
2706 425.2359 424.2286
2707 425.2360 424.2287
2708 425.2369 424.2296
2709 425.2896 424.2823
2710 425.2897 424.2824
2711 425.2899 424.2826
2712 425.2899 424.2826
2713 425.2903 424.2830
2714 426.0047 424.9974
2715 426.2737 425.2664
2716 427.0128 426.0055
2717 427.0135 426.0062
2718 427.0137 426.0064
2719 427.0140 426.0067
2720 427.1552 426.1479
2721 427.1566 426.1493
2722 427.1575 426.1502
2723 427.1961 426.1888
2724 427.1965 426.1892
2725 427.1967 426.1894
2726 427.1973 426.1900
2727 427.1985 426.1912
2728 427.2528 426.2455
2729 427.2692 426.2619
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