MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2
MS data file : PRT1311_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:18:13 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,460

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2801–2900 (out of 4256)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2530 411.9109 410.9036
2531 411.9112 410.9039
2532 413.0913 412.0840
2533 413.1405 412.1332
2534 413.1422 412.1349
2535 413.1813 412.1740
2536 413.1823 412.1750
2537 413.2686 412.2613
2538 413.2686 412.2613
2539 413.2687 412.2614
2540 413.2688 412.2615
2541 413.2689 412.2616
2542 413.2690 412.2617
2543 413.2693 412.2620
2544 413.2695 412.2622
2545 413.2698 412.2625
2546 413.2699 412.2626
2547 413.2701 412.2628
2548 413.2714 412.2641
2549 413.2731 412.2658
2550 414.2731 413.2658
2551 415.1556 414.1483
2552 415.1569 414.1496
2553 415.2135 414.2062
2554 415.2151 414.2078
2555 416.2638 415.2565
2556 416.2638 415.2565
2557 416.2644 415.2571
2558 417.0582 416.0509
2559 417.1905 416.1832
2560 417.1909 416.1836
2561 417.1915 416.1842
2562 417.8747 416.8674
2563 417.8753 416.8680
2564 417.8753 416.8680
2565 417.8754 416.8681
2566 419.1695 418.1622
2567 419.1696 418.1623
2568 419.1699 418.1626
2569 419.1699 418.1626
2570 419.1700 418.1627
2571 419.1703 418.1630
2572 419.1703 418.1630
2573 419.2053 418.1980
2574 419.2058 418.1985
2575 419.2062 418.1989
2576 419.2065 418.1992
2577 419.2068 418.1995
2578 419.2068 418.1995
2579 419.2071 418.1998
2580 419.2072 418.1999
2581 419.2433 418.2360
2582 419.3179 418.3106
2583 419.3179 418.3106
2584 419.3181 418.3108
2585 419.3181 418.3108
2586 419.3183 418.3110
2587 419.3184 418.3111
2588 419.3185 418.3112
2589 419.3185 418.3112
2590 419.3186 418.3113
2591 421.0602 420.0529
2592 421.0608 420.0535
2593 421.0610 420.0537
2594 421.0613 420.0540
2595 421.1471 420.1398
2596 421.1855 420.1782
2597 421.1856 420.1783
2598 421.1856 420.1783
2599 421.1856 420.1783
2600 421.1856 420.1783
2601 421.1857 420.1784
2602 421.1857 420.1784
2603 421.1857 420.1784
2604 421.1859 420.1786
2605 421.1859 420.1786
2606 421.1860 420.1787
2607 421.1862 420.1789
2608 421.1865 420.1792
2609 421.1870 420.1797
2610 421.2038 420.1965
2611 421.2041 420.1968
2612 421.2042 420.1969
2613 421.2042 420.1969
2614 421.2043 420.1970
2615 421.2043 420.1970
2616 421.2043 420.1970
2617 421.2045 420.1972
2618 421.2215 420.2142
2619 421.2220 420.2147
2620 421.2222 420.2149
2621 421.2224 420.2151
2622 421.2225 420.2152
2623 421.2225 420.2152
2624 421.2226 420.2153
2625 421.2227 420.2154
2626 421.2227 420.2154
2627 421.2227 420.2154
2628 421.2227 420.2154
2629 421.2227 420.2154
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