MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2
MS data file : PRT1311_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:18:13 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,460

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2701–2800 (out of 4256)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2430 407.2069 406.1996
2431 407.2070 406.1997
2432 407.2071 406.1998
2433 407.2072 406.1999
2434 407.2072 406.1999
2435 407.2072 406.1999
2436 407.2072 406.1999
2437 407.2075 406.2002
2438 407.2077 406.2004
2439 407.2100 406.2027
2440 407.2247 406.2174
2441 407.2247 406.2174
2442 407.2248 406.2175
2443 407.2248 406.2175
2444 407.2248 406.2175
2445 407.2249 406.2176
2446 407.2249 406.2176
2447 407.2249 406.2176
2448 407.2249 406.2176
2449 407.2250 406.2177
2450 407.2257 406.2184
2451 407.2260 406.2187
2452 407.2432 406.2359
2453 409.1459 408.1386
2454 409.1459 408.1386
2455 409.1486 408.1413
2456 409.1486 408.1413
2457 409.1490 408.1417
2458 409.1491 408.1418
2459 409.1496 408.1423
2460 409.1499 408.1426
2461 409.1500 408.1427
2462 409.1667 408.1594
2463 409.1765 408.1692
2464 409.1844 408.1771
2465 409.1849 408.1776
2466 409.1850 408.1777
2467 409.1856 408.1783
2468 409.1856 408.1783
2469 409.1857 408.1784
2470 409.1859 408.1786
2471 409.1861 408.1788
2472 409.1861 408.1788
2473 409.1861 408.1788
2474 409.1862 408.1789
2475 409.1862 408.1789
2476 409.1862 408.1789
2477 409.1862 408.1789
2478 409.1864 408.1791
2479 409.1866 408.1793
2480 409.2223 408.2150
2481 409.2223 408.2150
2482 409.2224 408.2151
2483 409.2226 408.2153
2484 409.2227 408.2154
2485 409.2227 408.2154
2486 409.2228 408.2155
2487 409.2229 408.2156
2488 409.2405 408.2332
2489 409.2406 408.2333
2490 409.2406 408.2333
2491 409.2406 408.2333
2492 409.2407 408.2334
2493 409.2418 408.2345
2494 409.2422 408.2349
2495 409.2744 408.2671
2496 409.2747 408.2674
2497 410.2435 409.2362
2498 410.2435 409.2362
2499 410.2435 409.2362
2500 410.2435 409.2362
2501 410.2927 409.2854
2502 411.0139 410.0066
2503 411.0147 410.0074
2504 411.0398 410.0325
2505 411.0983 410.0910
2506 411.1265 410.1192
2507 411.1628 410.1555
2508 411.1629 410.1556
2509 411.1634 410.1561
2510 411.1637 410.1564
2511 411.1647 410.1574
2512 411.1648 410.1575
2513 411.1649 410.1576
2514 411.1649 410.1576
2515 411.1655 410.1582
2516 411.1656 410.1583
2517 411.2006 410.1933
2518 411.2010 410.1937
2519 411.2014 410.1941
2520 411.2015 410.1942
2521 411.2018 410.1945
2522 411.2019 410.1946
2523 411.2020 410.1947
2524 411.2021 410.1948
2525 411.2021 410.1948
2526 411.2023 410.1950
2527 411.2025 410.1952
2528 411.2025 410.1952
2529 411.2577 410.2504
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