MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2
MS data file : PRT1311_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:18:13 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,460

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2601–2700 (out of 4256)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2330 397.1501 396.1428
2331 397.1854 396.1781
2332 397.1855 396.1782
2333 397.1858 396.1785
2334 397.1858 396.1785
2335 397.1860 396.1787
2336 397.1862 396.1789
2337 397.1862 396.1789
2338 397.1863 396.1790
2339 397.1863 396.1790
2340 397.1863 396.1790
2341 397.1865 396.1792
2342 397.1865 396.1792
2343 397.1866 396.1793
2344 397.1866 396.1793
2345 397.1866 396.1793
2346 397.2045 396.1972
2347 397.2224 396.2151
2348 397.2224 396.2151
2349 397.2226 396.2153
2350 397.2232 396.2159
2351 397.2404 396.2331
2352 398.2800 397.2727
2353 399.0760 398.0687
2354 399.1238 398.1165
2355 399.1258 398.1185
2356 399.1622 398.1549
2357 399.2220 398.2147
2358 401.1394 400.1321
2359 401.1414 400.1341
2360 401.1469 400.1396
2361 401.1578 400.1505
2362 401.2479 400.2406
2363 402.2473 401.2400
2364 402.2477 401.2404
2365 402.2480 401.2407
2366 403.0496 402.0423
2367 403.1749 402.1676
2368 403.1750 402.1677
2369 403.1751 402.1678
2370 403.1752 402.1679
2371 403.1754 402.1681
2372 403.1754 402.1681
2373 403.1758 402.1685
2374 403.2121 402.2048
2375 403.2313 402.2240
2376 403.2321 402.2248
2377 403.2322 402.2249
2378 403.2322 402.2249
2379 403.2324 402.2251
2380 403.2325 402.2252
2381 405.1535 404.1462
2382 405.1543 404.1470
2383 405.1545 404.1472
2384 405.1546 404.1473
2385 405.1549 404.1476
2386 405.1549 404.1476
2387 405.1902 404.1829
2388 405.1906 404.1833
2389 405.1906 404.1833
2390 405.1907 404.1834
2391 405.1908 404.1835
2392 405.1908 404.1835
2393 405.1908 404.1835
2394 405.1909 404.1836
2395 405.1909 404.1836
2396 405.1910 404.1837
2397 405.1910 404.1837
2398 405.1912 404.1839
2399 405.1914 404.1841
2400 405.1916 404.1843
2401 405.1928 404.1855
2402 405.2260 404.2187
2403 405.2269 404.2196
2404 405.2270 404.2197
2405 405.2272 404.2199
2406 405.2272 404.2199
2407 405.2278 404.2205
2408 405.2280 404.2207
2409 405.2292 404.2219
2410 406.1336 405.1263
2411 407.1697 406.1624
2412 407.1699 406.1626
2413 407.1699 406.1626
2414 407.1702 406.1629
2415 407.1702 406.1629
2416 407.1703 406.1630
2417 407.1703 406.1630
2418 407.1705 406.1632
2419 407.1705 406.1632
2420 407.1706 406.1633
2421 407.1707 406.1634
2422 407.1708 406.1635
2423 407.1709 406.1636
2424 407.1712 406.1639
2425 407.2063 406.1990
2426 407.2066 406.1993
2427 407.2068 406.1995
2428 407.2068 406.1995
2429 407.2068 406.1995
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