MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2
MS data file : PRT1311_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:18:13 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,460

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2501–2600 (out of 4256)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2230 393.2458 392.2385
2231 393.2459 392.2386
2232 393.2466 392.2393
2233 393.2990 392.2917
2234 393.2992 392.2919
2235 393.2994 392.2921
2236 393.2996 392.2923
2237 393.2997 392.2924
2238 393.2997 392.2924
2239 393.2997 392.2924
2240 393.2997 392.2924
2241 393.2997 392.2924
2242 393.2998 392.2925
2243 393.2998 392.2925
2244 393.2998 392.2925
2245 393.2998 392.2925
2246 393.2998 392.2925
2247 393.2998 392.2925
2248 393.2998 392.2925
2249 393.2998 392.2925
2250 393.2998 392.2925
2251 393.2998 392.2925
2252 393.2998 392.2925
2253 393.2999 392.2926
2254 393.2999 392.2926
2255 393.2999 392.2926
2256 393.2999 392.2926
2257 393.2999 392.2926
2258 393.2999 392.2926
2259 393.2999 392.2926
2260 393.2999 392.2926
2261 393.2999 392.2926
2262 393.2999 392.2926
2263 393.3000 392.2927
2264 393.3002 392.2929
2265 393.3002 392.2929
2266 393.3004 392.2931
2267 393.3008 392.2935
2268 395.0181 394.0108
2269 395.0367 394.0294
2270 395.0755 394.0682
2271 395.0764 394.0691
2272 395.1044 394.0971
2273 395.1153 394.1080
2274 395.1296 394.1223
2275 395.1298 394.1225
2276 395.1326 394.1253
2277 395.1673 394.1600
2278 395.1693 394.1620
2279 395.1695 394.1622
2280 395.1697 394.1624
2281 395.1701 394.1628
2282 395.1701 394.1628
2283 395.1701 394.1628
2284 395.1702 394.1629
2285 395.1702 394.1629
2286 395.1703 394.1630
2287 395.1703 394.1630
2288 395.1703 394.1630
2289 395.1704 394.1631
2290 395.1705 394.1632
2291 395.1705 394.1632
2292 395.1706 394.1633
2293 395.1707 394.1634
2294 395.1713 394.1640
2295 395.2062 394.1989
2296 395.2063 394.1990
2297 395.2063 394.1990
2298 395.2064 394.1991
2299 395.2067 394.1994
2300 395.2067 394.1994
2301 395.2068 394.1995
2302 395.2068 394.1995
2303 395.2068 394.1995
2304 395.2068 394.1995
2305 395.2069 394.1996
2306 395.2069 394.1996
2307 395.2070 394.1997
2308 395.2073 394.2000
2309 395.2073 394.2000
2310 395.2075 394.2002
2311 395.2248 394.2175
2312 395.2255 394.2182
2313 395.2265 394.2192
2314 395.2610 394.2537
2315 396.2640 395.2567
2316 396.2642 395.2569
2317 396.2650 395.2577
2318 396.8839 395.8766
2319 397.0515 396.0442
2320 397.0519 396.0446
2321 397.0522 396.0449
2322 397.0522 396.0449
2323 397.0962 396.0889
2324 397.1279 396.1206
2325 397.1444 396.1371
2326 397.1450 396.1377
2327 397.1461 396.1388
2328 397.1484 396.1411
2329 397.1487 396.1414
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