MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2
MS data file : PRT1311_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:18:13 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,460

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2401–2500 (out of 4256)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2130 391.1749 390.1676
2131 391.1750 390.1677
2132 391.1752 390.1679
2133 391.1752 390.1679
2134 391.1753 390.1680
2135 391.1754 390.1681
2136 391.1754 390.1681
2137 391.1754 390.1681
2138 391.1754 390.1681
2139 391.1755 390.1682
2140 391.1755 390.1682
2141 391.1756 390.1683
2142 391.1756 390.1683
2143 391.2114 390.2041
2144 391.2117 390.2044
2145 391.2117 390.2044
2146 391.2117 390.2044
2147 391.2118 390.2045
2148 391.2118 390.2045
2149 391.2119 390.2046
2150 391.2119 390.2046
2151 391.2119 390.2046
2152 391.2120 390.2047
2153 391.2120 390.2047
2154 391.2126 390.2053
2155 391.2128 390.2055
2156 391.2330 390.2257
2157 391.2479 390.2406
2158 391.2856 390.2783
2159 391.2857 390.2784
2160 391.2861 390.2788
2161 391.2863 390.2790
2162 391.2863 390.2790
2163 391.2864 390.2791
2164 391.2864 390.2791
2165 391.2864 390.2791
2166 391.2864 390.2791
2167 391.2864 390.2791
2168 391.2864 390.2791
2169 391.2864 390.2791
2170 391.2864 390.2791
2171 391.2865 390.2792
2172 391.2865 390.2792
2173 391.2865 390.2792
2174 391.2865 390.2792
2175 391.2865 390.2792
2176 391.2865 390.2792
2177 391.2865 390.2792
2178 391.2865 390.2792
2179 391.2865 390.2792
2180 391.2865 390.2792
2181 391.2866 390.2793
2182 391.2866 390.2793
2183 391.2866 390.2793
2184 391.2866 390.2793
2185 391.2867 390.2794
2186 391.2867 390.2794
2187 391.2867 390.2794
2188 391.2867 390.2794
2189 391.2868 390.2795
2190 391.2871 390.2798
2191 391.2876 390.2803
2192 393.0898 392.0825
2193 393.1529 392.1456
2194 393.1532 392.1459
2195 393.1538 392.1465
2196 393.1543 392.1470
2197 393.1543 392.1470
2198 393.1546 392.1473
2199 393.1547 392.1474
2200 393.1548 392.1475
2201 393.1548 392.1475
2202 393.1548 392.1475
2203 393.1548 392.1475
2204 393.1550 392.1477
2205 393.1909 392.1836
2206 393.1909 392.1836
2207 393.1909 392.1836
2208 393.1909 392.1836
2209 393.1909 392.1836
2210 393.1910 392.1837
2211 393.1910 392.1837
2212 393.1911 392.1838
2213 393.1911 392.1838
2214 393.1911 392.1838
2215 393.1912 392.1839
2216 393.1912 392.1839
2217 393.1913 392.1840
2218 393.1914 392.1841
2219 393.1915 392.1842
2220 393.1917 392.1844
2221 393.1921 392.1848
2222 393.2119 392.2046
2223 393.2267 392.2194
2224 393.2273 392.2200
2225 393.2274 392.2201
2226 393.2276 392.2203
2227 393.2450 392.2377
2228 393.2456 392.2383
2229 393.2458 392.2385
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