MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2
MS data file : PRT1311_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:18:13 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,460

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2301–2400 (out of 4256)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2030 381.1911 380.1838
2031 381.1911 380.1838
2032 381.1911 380.1838
2033 381.1912 380.1839
2034 381.1915 380.1842
2035 381.1915 380.1842
2036 381.2092 380.2019
2037 381.2095 380.2022
2038 381.2097 380.2024
2039 381.2098 380.2025
2040 381.2099 380.2026
2041 381.2099 380.2026
2042 381.2102 380.2029
2043 381.2103 380.2030
2044 381.2103 380.2030
2045 381.2104 380.2031
2046 381.2105 380.2032
2047 381.2106 380.2033
2048 381.2123 380.2050
2049 381.2461 380.2388
2050 382.9865 381.9792
2051 383.1032 382.0959
2052 383.1034 382.0961
2053 383.1035 382.0962
2054 383.1302 382.1229
2055 383.1305 382.1232
2056 383.1581 382.1508
2057 383.1693 382.1620
2058 383.1694 382.1621
2059 383.1695 382.1622
2060 383.1696 382.1623
2061 383.1703 382.1630
2062 383.1703 382.1630
2063 383.1705 382.1632
2064 383.1706 382.1633
2065 383.1707 382.1634
2066 383.1708 382.1635
2067 383.1709 382.1636
2068 383.1711 382.1638
2069 383.1715 382.1642
2070 383.2070 382.1997
2071 383.2071 382.1998
2072 383.2071 382.1998
2073 383.2256 382.2183
2074 383.2262 382.2189
2075 383.2262 382.2189
2076 383.2263 382.2190
2077 383.2264 382.2191
2078 383.2264 382.2191
2079 383.2265 382.2192
2080 383.2267 382.2194
2081 383.2270 382.2197
2082 383.2270 382.2197
2083 383.2270 382.2197
2084 385.1449 384.1376
2085 385.1460 384.1387
2086 385.1462 384.1389
2087 385.1875 384.1802
2088 385.2208 384.2135
2089 385.2216 384.2143
2090 385.2423 384.2350
2091 385.2426 384.2353
2092 385.2430 384.2357
2093 385.2436 384.2363
2094 386.0239 385.0166
2095 387.0563 386.0490
2096 387.1248 386.1175
2097 387.1259 386.1186
2098 387.1796 386.1723
2099 387.1799 386.1726
2100 387.1805 386.1732
2101 387.1813 386.1740
2102 387.1961 386.1888
2103 387.2762 386.2689
2104 387.2762 386.2689
2105 389.1591 388.1518
2106 389.1592 388.1519
2107 389.1598 388.1525
2108 389.1608 388.1535
2109 389.1955 388.1882
2110 389.1958 388.1885
2111 389.1959 388.1886
2112 389.1962 388.1889
2113 389.1966 388.1893
2114 389.1966 388.1893
2115 389.1966 388.1893
2116 389.1968 388.1895
2117 389.2524 388.2451
2118 389.2525 388.2452
2119 389.2528 388.2455
2120 389.2531 388.2458
2121 389.2532 388.2459
2122 390.1399 389.1326
2123 391.1364 390.1291
2124 391.1380 390.1307
2125 391.1383 390.1310
2126 391.1385 390.1312
2127 391.1385 390.1312
2128 391.1386 390.1313
2129 391.1392 390.1319
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