MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2
MS data file : PRT1311_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:18:13 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,460

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2201–2300 (out of 4256)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1930 377.1596 376.1523
1931 377.1597 376.1524
1932 377.1598 376.1525
1933 377.1598 376.1525
1934 377.1600 376.1527
1935 377.1601 376.1528
1936 377.1601 376.1528
1937 377.1958 376.1885
1938 377.1958 376.1885
1939 377.1959 376.1886
1940 377.1959 376.1886
1941 377.1960 376.1887
1942 377.1960 376.1887
1943 377.1960 376.1887
1944 377.1960 376.1887
1945 377.1960 376.1887
1946 377.1962 376.1889
1947 377.1962 376.1889
1948 377.1963 376.1890
1949 377.1963 376.1890
1950 377.1964 376.1891
1951 377.8613 376.8540
1952 378.3026 377.2953
1953 378.9664 377.9591
1954 379.1349 378.1276
1955 379.1376 378.1303
1956 379.1379 378.1306
1957 379.1381 378.1308
1958 379.1386 378.1313
1959 379.1388 378.1315
1960 379.1389 378.1316
1961 379.1390 378.1317
1962 379.1390 378.1317
1963 379.1747 378.1674
1964 379.1749 378.1676
1965 379.1751 378.1678
1966 379.1751 378.1678
1967 379.1752 378.1679
1968 379.1752 378.1679
1969 379.1752 378.1679
1970 379.1752 378.1679
1971 379.1752 378.1679
1972 379.1752 378.1679
1973 379.1752 378.1679
1974 379.1753 378.1680
1975 379.1753 378.1680
1976 379.1753 378.1680
1977 379.1754 378.1681
1978 379.1754 378.1681
1979 379.1754 378.1681
1980 379.1755 378.1682
1981 379.1755 378.1682
1982 379.1756 378.1683
1983 379.1936 378.1863
1984 379.1936 378.1863
1985 379.1939 378.1866
1986 379.1942 378.1869
1987 379.2110 378.2037
1988 379.2114 378.2041
1989 379.2117 378.2044
1990 379.2117 378.2044
1991 379.2118 378.2045
1992 379.2118 378.2045
1993 379.2119 378.2046
1994 379.2119 378.2046
1995 379.2120 378.2047
1996 379.2121 378.2048
1997 379.2122 378.2049
1998 379.2126 378.2053
1999 379.2645 378.2572
2000 381.1164 380.1091
2001 381.1516 380.1443
2002 381.1521 380.1448
2003 381.1532 380.1459
2004 381.1537 380.1464
2005 381.1542 380.1469
2006 381.1542 380.1469
2007 381.1543 380.1470
2008 381.1544 380.1471
2009 381.1544 380.1471
2010 381.1547 380.1474
2011 381.1548 380.1475
2012 381.1548 380.1475
2013 381.1548 380.1475
2014 381.1549 380.1476
2015 381.1898 380.1825
2016 381.1900 380.1827
2017 381.1904 380.1831
2018 381.1907 380.1834
2019 381.1908 380.1835
2020 381.1909 380.1836
2021 381.1909 380.1836
2022 381.1909 380.1836
2023 381.1909 380.1836
2024 381.1909 380.1836
2025 381.1909 380.1836
2026 381.1910 380.1837
2027 381.1910 380.1837
2028 381.1910 380.1837
2029 381.1910 380.1837
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