MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2
MS data file : PRT1311_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:18:13 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,460

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2101–2200 (out of 4256)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1830 371.0815 370.0742
1831 371.0932 370.0859
1832 371.1044 370.0971
1833 371.1295 370.1222
1834 371.1309 370.1236
1835 371.1315 370.1242
1836 371.1369 370.1296
1837 371.1404 370.1331
1838 371.1715 370.1642
1839 371.1716 370.1643
1840 371.1859 370.1786
1841 371.1862 370.1789
1842 371.1877 370.1804
1843 371.1877 370.1804
1844 371.2302 370.2229
1845 371.2422 370.2349
1846 371.2647 370.2574
1847 371.3033 370.2960
1848 371.3034 370.2961
1849 371.3172 370.3099
1850 371.3172 370.3099
1851 371.3172 370.3099
1852 371.3173 370.3100
1853 371.3174 370.3101
1854 371.3174 370.3101
1855 371.3174 370.3101
1856 371.3174 370.3101
1857 371.3174 370.3101
1858 371.3174 370.3101
1859 371.3175 370.3102
1860 371.3175 370.3102
1861 371.3175 370.3102
1862 371.3175 370.3102
1863 371.3175 370.3102
1864 371.3175 370.3102
1865 371.3175 370.3102
1866 371.3175 370.3102
1867 371.3176 370.3103
1868 371.3176 370.3103
1869 371.3176 370.3103
1870 371.3176 370.3103
1871 371.3176 370.3103
1872 371.3177 370.3104
1873 371.3177 370.3104
1874 371.3177 370.3104
1875 371.3177 370.3104
1876 371.3177 370.3104
1877 371.3178 370.3105
1878 371.3178 370.3105
1879 371.3178 370.3105
1880 371.3179 370.3106
1881 371.3179 370.3106
1882 371.3180 370.3107
1883 371.3186 370.3113
1884 372.0522 371.0449
1885 373.0940 372.0867
1886 373.0941 372.0868
1887 373.0944 372.0871
1888 374.0000 372.9927
1889 374.1662 373.1589
1890 374.1664 373.1591
1891 374.1664 373.1591
1892 374.1673 373.1600
1893 375.0673 374.0600
1894 375.0678 374.0605
1895 375.1410 374.1337
1896 375.1431 374.1358
1897 375.1432 374.1359
1898 375.1436 374.1363
1899 375.1436 374.1363
1900 375.1437 374.1364
1901 375.1440 374.1367
1902 375.1440 374.1367
1903 375.1441 374.1368
1904 375.1798 374.1725
1905 375.1802 374.1729
1906 375.1803 374.1730
1907 375.1803 374.1730
1908 375.1805 374.1732
1909 375.1807 374.1734
1910 375.2009 374.1936
1911 375.2164 374.2091
1912 375.2164 374.2091
1913 375.2180 374.2107
1914 375.8549 374.8476
1915 375.8633 374.8560
1916 375.8639 374.8566
1917 375.8639 374.8566
1918 377.1210 376.1137
1919 377.1566 376.1493
1920 377.1576 376.1503
1921 377.1588 376.1515
1922 377.1592 376.1519
1923 377.1593 376.1520
1924 377.1594 376.1521
1925 377.1595 376.1522
1926 377.1595 376.1522
1927 377.1595 376.1522
1928 377.1595 376.1522
1929 377.1595 376.1522
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