MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2
MS data file : PRT1311_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:18:13 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,460

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2001–2100 (out of 4256)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1730 367.1750 366.1677
1731 367.1752 366.1679
1732 367.1752 366.1679
1733 367.1753 366.1680
1734 367.1754 366.1681
1735 367.1754 366.1681
1736 367.1754 366.1681
1737 367.1754 366.1681
1738 367.1754 366.1681
1739 367.1754 366.1681
1740 367.1754 366.1681
1741 367.1755 366.1682
1742 367.1755 366.1682
1743 367.1755 366.1682
1744 367.1755 366.1682
1745 367.1755 366.1682
1746 367.1756 366.1683
1747 367.1756 366.1683
1748 367.1927 366.1854
1749 367.1929 366.1856
1750 367.1930 366.1857
1751 367.1931 366.1858
1752 367.1931 366.1858
1753 367.1933 366.1860
1754 367.1937 366.1864
1755 367.1938 366.1865
1756 367.2094 366.2021
1757 367.2116 366.2043
1758 367.2116 366.2043
1759 367.2117 366.2044
1760 367.2117 366.2044
1761 367.2118 366.2045
1762 367.2120 366.2047
1763 367.2123 366.2050
1764 367.2136 366.2063
1765 367.2318 366.2245
1766 367.2319 366.2246
1767 367.2320 366.2247
1768 367.2320 366.2247
1769 367.2321 366.2248
1770 367.2321 366.2248
1771 367.2321 366.2248
1772 367.2321 366.2248
1773 367.2325 366.2252
1774 367.2325 366.2252
1775 367.2336 366.2263
1776 368.2309 367.2236
1777 368.2314 367.2241
1778 368.9293 367.9220
1779 369.1134 368.1061
1780 369.1137 368.1064
1781 369.1152 368.1079
1782 369.1156 368.1083
1783 369.1523 368.1450
1784 369.1535 368.1462
1785 369.1539 368.1466
1786 369.1543 368.1470
1787 369.1544 368.1471
1788 369.1546 368.1473
1789 369.1548 368.1475
1790 369.1549 368.1476
1791 369.1552 368.1479
1792 369.1552 368.1479
1793 369.1552 368.1479
1794 369.1705 368.1632
1795 369.1705 368.1632
1796 369.1717 368.1644
1797 369.1717 368.1644
1798 369.1720 368.1647
1799 369.1724 368.1651
1800 369.1914 368.1841
1801 369.1914 368.1841
1802 369.1918 368.1845
1803 369.2090 368.2017
1804 369.2092 368.2019
1805 369.2103 368.2030
1806 369.2264 368.2191
1807 369.2478 368.2405
1808 369.2483 368.2410
1809 369.2654 368.2581
1810 370.0290 369.0217
1811 370.0290 369.0217
1812 370.0290 369.0217
1813 370.0291 369.0218
1814 370.0292 369.0219
1815 370.8843 369.8770
1816 370.8844 369.8771
1817 370.8844 369.8771
1818 370.8844 369.8771
1819 370.8844 369.8771
1820 370.8845 369.8772
1821 370.8845 369.8772
1822 370.8846 369.8773
1823 370.8846 369.8773
1824 370.8846 369.8773
1825 370.8847 369.8774
1826 370.8851 369.8778
1827 371.0797 370.0724
1828 371.0801 370.0728
1829 371.0811 370.0738
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