MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2
MS data file : PRT1311_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:18:13 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,460

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1901–2000 (out of 4256)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1630 363.1800 362.1727
1631 363.1800 362.1727
1632 363.1800 362.1727
1633 363.1801 362.1728
1634 363.1801 362.1728
1635 363.1801 362.1728
1636 363.1801 362.1728
1637 363.1801 362.1728
1638 363.1802 362.1729
1639 363.1802 362.1729
1640 363.1802 362.1729
1641 363.1803 362.1730
1642 363.1803 362.1730
1643 363.1803 362.1730
1644 363.1804 362.1731
1645 363.1804 362.1731
1646 363.1805 362.1732
1647 363.1805 362.1732
1648 363.2161 362.2088
1649 363.2165 362.2092
1650 363.2168 362.2095
1651 363.2177 362.2104
1652 364.9939 363.9866
1653 364.9940 363.9867
1654 364.9943 363.9870
1655 364.9945 363.9872
1656 365.1194 364.1121
1657 365.1213 364.1140
1658 365.1214 364.1141
1659 365.1215 364.1142
1660 365.1221 364.1148
1661 365.1226 364.1153
1662 365.1565 364.1492
1663 365.1582 364.1509
1664 365.1589 364.1516
1665 365.1592 364.1519
1666 365.1592 364.1519
1667 365.1593 364.1520
1668 365.1594 364.1521
1669 365.1594 364.1521
1670 365.1594 364.1521
1671 365.1594 364.1521
1672 365.1595 364.1522
1673 365.1595 364.1522
1674 365.1595 364.1522
1675 365.1595 364.1522
1676 365.1595 364.1522
1677 365.1595 364.1522
1678 365.1596 364.1523
1679 365.1596 364.1523
1680 365.1598 364.1525
1681 365.1956 364.1883
1682 365.1957 364.1884
1683 365.1958 364.1885
1684 365.1959 364.1886
1685 365.1959 364.1886
1686 365.1959 364.1886
1687 365.1959 364.1886
1688 365.1959 364.1886
1689 365.1960 364.1887
1690 365.1960 364.1887
1691 365.1960 364.1887
1692 365.1960 364.1887
1693 365.1960 364.1887
1694 365.1960 364.1887
1695 365.1960 364.1887
1696 365.1961 364.1888
1697 365.1961 364.1888
1698 365.1961 364.1888
1699 365.1961 364.1888
1700 365.1969 364.1896
1701 365.1971 364.1898
1702 365.1975 364.1902
1703 365.2150 364.2077
1704 365.2153 364.2080
1705 365.2154 364.2081
1706 365.2155 364.2082
1707 365.2156 364.2083
1708 365.2157 364.2084
1709 365.2158 364.2085
1710 365.2160 364.2087
1711 365.2161 364.2088
1712 365.2162 364.2089
1713 365.2162 364.2089
1714 365.2165 364.2092
1715 367.1351 366.1278
1716 367.1361 366.1288
1717 367.1365 366.1292
1718 367.1381 366.1308
1719 367.1383 366.1310
1720 367.1384 366.1311
1721 367.1385 366.1312
1722 367.1386 366.1313
1723 367.1386 366.1313
1724 367.1386 366.1313
1725 367.1387 366.1314
1726 367.1388 366.1315
1727 367.1740 366.1667
1728 367.1745 366.1672
1729 367.1750 366.1677
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