MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Andy2 |
| MS data file | : | PRT1311_JBEI_2.mgf |
| Database | : | Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues) |
| Timestamp | : | 8 Sep 2025 at 17:18:13 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,460 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
|---|---|---|---|---|---|---|---|---|---|
| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
| 3916 |
618.3326 | 1851.9760 | 1851.9479 | 15.2 | 1 | 4 | 1.5 | 1Score > 19 indicates identity |
QAQLPGKLFTQNIGAHE + Deamidated (NQ) |
| 4216 |
833.7418 | 2498.2036 | 2498.1875 | 6.43 | 1 | 4 | 1.6 | 1Score > 19 indicates identity |
DKLFNLPFNMNTFHQMWGVK + 2 Oxidation (M) |
| 3575 |
602.8173 | 1203.6200 | 1203.6135 | 5.41 | 1 | 4 | 0.81 | 1Score > 21 indicates identity Score > 16 indicates homology |
ELFDPIISDR |
| 3858 |
582.6255 | 1744.8547 | 1744.8883 | -19.3 | 1 | 4 | 1 | 1Score > 20 indicates identity Score > 17 indicates homology |
TPFQIAELNAQAQLAK + 3 Deamidated (NQ) |
| 3749 |
721.8704 | 1441.7262 | 1441.7049 | 14.8 | 1 | 4 | 1 | 1Score > 20 indicates identity Score > 17 indicates homology |
LQKDQNGADIPDK + Deamidated (NQ) |
| 3782 |
384.7240 | 1534.8669 | 1534.8759 | -5.87 | 1 | 4 | 0.49 | 1Score > 14 indicates identity |
VLNVLGIFQEYIK |
| 3731 |
712.8618 | 1423.7090 | 1423.7096 | -0.38 | 1 | 4 | 0.56 | 1Score > 20 indicates identity Score > 14 indicates homology |
DIEVHVFPDTPR |
| 3635 |
419.5560 | 1255.6462 | 1255.6520 | -4.66 | 1 | 4 | 0.56 | 1Score > 20 indicates identity Score > 14 indicates homology |
RQQALQQALAE + Deamidated (NQ) |
| 3952 |
652.3372 | 1953.9898 | 1953.9553 | 17.6 | 0 | 4 | 1.9 | 1Score > 20 indicates identity |
VIGHLNTMHTISSMAWR + Deamidated (NQ) |
| 3011 |
488.2851 | 487.2778 | 487.2867 | -18.1 | 1 | 4 | 1 | 1Score > 27 indicates identity Score > 17 indicates homology |
GGKAR |
| 3832 |
567.9709 | 1700.8909 | 1700.8668 | 14.1 | 1 | 4 | 1.9 | 1Score > 19 indicates identity |
RDFPHLTMSINGGIK + Oxidation (M) |
| 4210 |
832.0815 | 2493.2227 | 2493.2646 | -16.8 | 1 | 4 | 1.2 | 1Score > 17 indicates identity |
TGSLNLYVMDLASGQIRQVTDGR |
| 3552 |
583.8214 | 1165.6282 | 1165.6455 | -14.8 | 0 | 4 | 1.6 | 1Score > 19 indicates identity |
LQDIPPSQIR |
| 3810 |
414.7298 | 1654.8901 | 1654.8638 | 15.9 | 1 | 4 | 1.5 | 1Score > 18 indicates identity |
SPNDKLNSNLNLVAR + Deamidated (NQ) |
| 3816 |
419.2317 | 1672.8977 | 1672.8784 | 11.5 | 1 | 4 | 1.7 | 1Score > 19 indicates identity |
DYSHLTNDLVGAIKK |
| 3676 |
434.5557 | 1300.6453 | 1300.6510 | -4.42 | 1 | 4 | 0.72 | 1Score > 21 indicates identity Score > 15 indicates homology |
INGQAQILEGQK + 3 Deamidated (NQ) |
| 3597 |
307.8998 | 1227.5701 | 1227.5632 | 5.58 | 0 | 4 | 1.6 | 1Score > 18 indicates identity |
NQTGIHTDWR + Deamidated (NQ) |
| 4078 |
457.2539 | 2281.2331 | 2281.2008 | 14.2 | 1 | 4 | 0.92 | 1Score > 16 indicates identity |
VAFTWQGGEPTLLGLAFYRR |
| 3729 |
356.6689 | 1422.6465 | 1422.6489 | -1.69 | 1 | 4 | 0.73 | 1Score > 20 indicates identity Score > 15 indicates homology |
MYRYLAIANYE + Deamidated (NQ); Oxidation (M) |
| 3970 |
687.0180 | 2058.0322 | 2058.0190 | 6.38 | 0 | 4 | 2.2 | 1Score > 19 indicates identity |
ANAATGNLNILLPMVTSLDE + 2 Deamidated (NQ) |
| 3560 |
396.5462 | 1186.6168 | 1186.6054 | 9.59 | 1 | 3 | 1 | 1Score > 21 indicates identity Score > 16 indicates homology |
NLAQRSAQAAR + 2 Deamidated (NQ) |
| 3659 |
425.8708 | 1274.5906 | 1274.5931 | -2.01 | 1 | 3 | 0.95 | 1Score > 20 indicates identity Score > 16 indicates homology |
AEQLFDFSYR |
| 3162 |
316.6628 | 631.3110 | 631.3177 | -10.5 | 0 | 3 | 1 | 1Score > 22 indicates identity Score > 16 indicates homology |
NGSILE |
| 3779 |
378.7003 | 1510.7721 | 1510.7569 | 10.1 | 1 | 3 | 2.2 | 1Score > 19 indicates identity |
FEPIRPYEFTGR |
| 3795 |
392.9577 | 1567.8017 | 1567.7704 | 19.9 | 0 | 3 | 0.64 | 1Score > 21 indicates identity Score > 14 indicates homology |
NFNLAMINPIFTR + 2 Deamidated (NQ); Oxidation (M) |
| 3551 |
583.8213 | 1165.6280 | 1165.6455 | -15.0 | 0 | 3 | 1.9 | 1Score > 19 indicates identity |
QLLNLRPSPE |
| 3848 |
575.9874 | 1724.9404 | 1724.9097 | 17.8 | 1 | 3 | 1.4 | 1Score > 17 indicates identity |
GFIASVVIDNKTFTGR + Deamidated (NQ) |
| 3496 |
542.8126 | 1083.6106 | 1083.5924 | 16.8 | 0 | 3 | 1.8 | 1Score > 18 indicates identity |
QIQLPSLQR + 2 Deamidated (NQ) |
| 3671 |
647.8401 | 1293.6656 | 1293.6565 | 7.09 | 0 | 3 | 2.3 | 1Score > 19 indicates identity |
AVADQTATAGYVK |
| 4089 |
457.4542 | 2282.2346 | 2282.2787 | -19.3 | 0 | 3 | 0.95 | 1Score > 15 indicates identity |
GWAIPAATDIAFALGVLALLGSR |
| 4004 |
720.3491 | 2158.0255 | 2158.0407 | -7.05 | 1 | 3 | 2.2 | 1Score > 19 indicates identity |
RLMQMMPQVLPSPDAMGPK + 2 Oxidation (M) |
| 3324 |
435.7752 | 869.5358 | 869.5334 | 2.78 | 1 | 3 | 1.6 | 1Score > 18 indicates identity |
VVAAIIER |
| 3661 |
427.2466 | 1278.7180 | 1278.7044 | 10.6 | 0 | 3 | 1.2 | 1Score > 16 indicates identity |
IAAVIGNHDTLR |
| 3364 |
946.4894 | 945.4821 | 945.4920 | -10.4 | 1 | 3 | 0.8 | 1Score > 22 indicates identity Score > 15 indicates homology |
SEWANLVK |
| 4290 |
935.1663 | 2802.4771 | 2802.4585 | 6.62 | 1 | 3 | 1.1 | 1Score > 16 indicates identity |
ASQFAAIASALLINVGTLTQPRAQAMR + 4 Deamidated (NQ) |
| 3415 |
496.2475 | 990.4804 | 990.4804 | 0.040 | 1 | 3 | 0.85 | 1Score > 21 indicates identity Score > 15 indicates homology |
LGAEVMSQR + Deamidated (NQ) |
| 3831 |
566.9599 | 1697.8579 | 1697.8485 | 5.51 | 0 | 3 | 0.78 | 1Score > 20 indicates identity Score > 14 indicates homology |
SNHNFLVQAGNVQLR + 2 Deamidated (NQ) |
| 4117 |
578.5653 | 2310.2321 | 2310.2406 | -3.68 | 1 | 3 | 1.7 | 1Score > 18 indicates identity |
KPGLTLGIGCETAQFPLPQVGK |
| 3727 |
707.3559 | 1412.6972 | 1412.7082 | -7.74 | 0 | 3 | 1 | 1Score > 20 indicates identity Score > 15 indicates homology |
GGDPALCQAALAAAK |
| 3873 |
441.7287 | 1762.8857 | 1762.8625 | 13.2 | 1 | 3 | 0.76 | 1Score > 20 indicates identity Score > 14 indicates homology |
AYNAIVDNNVELVSIE + Deamidated (NQ) |
| 4204 |
826.4181 | 2476.2325 | 2476.2274 | 2.04 | 1 | 3 | 2.3 | 1Score > 19 indicates identity |
NKSGQDSVPLLGDIPWFGQLFR + 3 Deamidated (NQ) |
| 4047 |
738.0428 | 2211.1066 | 2211.1465 | -18.1 | 1 | 3 | 2.2 | 1Score > 19 indicates identity |
VTAMLFSMAVGLNAVSMAAKAK + Deamidated (NQ) |
| 4186 |
818.7469 | 2453.2189 | 2453.1710 | 19.5 | 1 | 3 | 2.1 | 1Score > 19 indicates identity |
LAQIDQHIADGELNLWQASSDK + 2 Deamidated (NQ) |
| 3972 |
687.3522 | 2059.0348 | 2059.0585 | -11.5 | 1 | 3 | 0.85 | 1Score > 20 indicates identity Score > 14 indicates homology |
ADKLGYNLVVLDSQNNPAK + Deamidated (NQ) |
| 3992 |
536.5284 | 2142.0845 | 2142.0602 | 11.4 | 1 | 3 | 2.1 | 1Score > 18 indicates identity |
YALVTMCIGGGQGIAAIFER + Oxidation (M) |
| 3919 |
466.4888 | 1861.9261 | 1861.9621 | -19.3 | 1 | 3 | 1 | 1Score > 20 indicates identity Score > 15 indicates homology |
TGKPIYNAIVWQCRR + Deamidated (NQ) |
| 3555 |
589.8148 | 1177.6150 | 1177.6091 | 5.05 | 1 | 3 | 1 | 1Score > 21 indicates identity Score > 15 indicates homology |
DNAIAYKVQR + Deamidated (NQ) |
| 3355 |
466.7646 | 931.5146 | 931.5226 | -8.52 | 1 | 3 | 0.96 | 1Score > 20 indicates identity Score > 15 indicates homology |
ITLEQLSK + Deamidated (NQ) |
| 4045 |
737.7197 | 2210.1373 | 2210.1735 | -16.4 | 0 | 3 | 1.8 | 1Score > 17 indicates identity |
YPFTAITGVNTLFNALLNNK |
| 4375 |
1094.2616 | 3279.7630 | 3279.7213 | 12.7 | 1 | 3 | 0.76 | 1Score > 14 indicates identity |
WGVGLTFLLAATSVMAKDIQLLNVSYDPTR + Deamidated (NQ) |
| 4088 |
571.5659 | 2282.2345 | 2282.2787 | -19.4 | 0 | 2 | 1.1 | 1Score > 15 indicates identity |
GWAIPAATDIAFALGVLALLGSR |
| 4077 |
571.3152 | 2281.2317 | 2281.2008 | 13.6 | 1 | 2 | 1.2 | 1Score > 16 indicates identity |
VAFTWQGGEPTLLGLAFYRR |
| 3868 |
584.9854 | 1751.9344 | 1751.9352 | -0.48 | 1 | 2 | 1.8 | 1Score > 17 indicates identity |
LATHAGQIMDRDALLK |
| 3374 |
478.2852 | 954.5558 | 954.5651 | -9.65 | 1 | 2 | 1 | 1Score > 15 indicates identity |
VPALHKYK |
| 3648 |
633.3267 | 1264.6388 | 1264.6452 | -4.99 | 1 | 2 | 1 | 1Score > 21 indicates identity Score > 15 indicates homology |
IALPGYAFQRE + Deamidated (NQ) |
| 3144 |
309.6725 | 617.3304 | 617.3319 | -2.34 | 0 | 2 | 0.71 | 1Score > 21 indicates identity Score > 13 indicates homology |
MKPAR + Oxidation (M) |
| 3592 |
613.3525 | 1224.6904 | 1224.6826 | 6.37 | 0 | 2 | 1.1 | 1Score > 15 indicates identity |
GTGIPVIADGGIR |
| 3611 |
412.2339 | 1233.6799 | 1233.6578 | 17.9 | 1 | 2 | 1.6 | 1Score > 17 indicates identity |
NPRHQQALLR + 2 Deamidated (NQ) |
| 4338 |
982.5291 | 2944.5655 | 2944.5328 | 11.1 | 1 | 2 | 1.1 | 1Score > 15 indicates identity |
VAVARALLNPCSLLLLDEPAASLDAHSE |
| 4257 |
665.5793 | 2658.2881 | 2658.2822 | 2.22 | 0 | 2 | 1.9 | 1Score > 18 indicates identity |
MSGSNNFAGLSPVMNLLDVPFLFR + Deamidated (NQ); 2 Oxidation (M) |
| 4092 |
571.5662 | 2282.2357 | 2282.2787 | -18.8 | 0 | 2 | 1.1 | 1Score > 15 indicates identity |
GWAIPAATDIAFALGVLALLGSR |
| 3869 |
877.4731 | 1752.9316 | 1752.9159 | 8.99 | 0 | 2 | 1.9 | 1Score > 17 indicates identity |
QAVISPSWSIHSGVGTK |
| 4121 |
585.8213 | 2339.2561 | 2339.2235 | 13.9 | 0 | 2 | 1.2 | 1Score > 16 indicates identity |
WPLMQGDYLSSLILAIYALR + Deamidated (NQ); Oxidation (M) |
| 4070 |
756.0516 | 2265.1330 | 2265.1239 | 4.00 | 0 | 2 | 2.8 | 1Score > 19 indicates identity |
NFGNVVLVMFVTGPVQQTALE + 3 Deamidated (NQ) |
| 4215 |
833.7415 | 2498.2027 | 2498.1875 | 6.07 | 1 | 2 | 2.6 | 1Score > 19 indicates identity |
DKLFNLPFNMNTFHQMWGVK + 2 Oxidation (M) |
| 3431 |
504.2465 | 1006.4784 | 1006.4607 | 17.6 | 0 | 2 | 0.94 | 1Score > 21 indicates identity Score > 14 indicates homology |
LQNIFNQE + 2 Deamidated (NQ) |
| 4001 |
540.3055 | 2157.1929 | 2157.2350 | -19.5 | 0 | 2 | 0.81 | 1Score > 14 indicates identity |
YIGAVAILWVLAIGFNPALR + Deamidated (NQ) |
| 4130 |
781.4220 | 2341.2442 | 2341.2001 | 18.8 | 1 | 2 | 1.6 | 1Score > 17 indicates identity |
VLALYIATPRQWATHNMSPR + Deamidated (NQ); Oxidation (M) |
| 3674 |
432.2486 | 1293.7240 | 1293.7115 | 9.66 | 1 | 2 | 1.3 | 1Score > 16 indicates identity |
ILGAKASCISFK |
| 3132 |
304.1646 | 606.3146 | 606.3159 | -2.09 | 0 | 2 | 1 | 1Score > 21 indicates identity Score > 15 indicates homology |
MTALR + Oxidation (M) |
| 3522 |
372.8858 | 1115.6356 | 1115.6372 | -1.49 | 1 | 2 | 1.8 | 1Score > 17 indicates identity |
MRLLQTLIE |
| 3174 |
321.1918 | 640.3690 | 640.3656 | 5.34 | 0 | 2 | 1.3 | 1Score > 16 indicates identity |
APNIAR |
| 3422 |
499.7257 | 997.4368 | 997.4175 | 19.4 | 0 | 2 | 2.5 | 1Score > 18 indicates identity |
MTPSNYQR + 2 Deamidated (NQ) |
| 3771 |
498.5863 | 1492.7371 | 1492.7310 | 4.05 | 1 | 2 | 0.72 | 1Score > 20 indicates identity Score > 13 indicates homology |
IESYQGAAGGWGAVK |
| 3369 |
474.2820 | 946.5494 | 946.5633 | -14.7 | 1 | 2 | 1 | 1Score > 15 indicates identity |
MKIAILSR + Oxidation (M) |
| 4086 |
761.7519 | 2282.2339 | 2282.2508 | -7.43 | 1 | 2 | 1.2 | 1Score > 15 indicates identity |
ILIVEDDTLLLQGLILAAQTE + 2 Deamidated (NQ) |
| 4255 |
665.5782 | 2658.2837 | 2658.2822 | 0.56 | 0 | 2 | 2.1 | 1Score > 18 indicates identity |
MSGSNNFAGLSPVMNLLDVPFLFR + Deamidated (NQ); 2 Oxidation (M) |
| 3433 |
505.2318 | 1008.4490 | 1008.4442 | 4.78 | 0 | 2 | 3.1 | 1Score > 19 indicates identity |
CVMLMLNE |
| 3515 |
550.8042 | 1099.5938 | 1099.5848 | 8.21 | 0 | 2 | 0.98 | 1Score > 20 indicates identity Score > 14 indicates homology |
IAWMPQGLGK |
| 3517 |
369.8773 | 1106.6101 | 1106.6237 | -12.3 | 1 | 2 | 2.5 | 1Score > 18 indicates identity |
QKYFPIVGR |
| 3856 |
577.9825 | 1730.9257 | 1730.8992 | 15.3 | 1 | 2 | 2.8 | 1Score > 19 indicates identity |
ATSGAKAFVFQSVYAGK |
| 3631 |
627.8416 | 1253.6686 | 1253.6615 | 5.67 | 1 | 2 | 2.8 | 1Score > 19 indicates identity |
ADGNGIVLLPER + Deamidated (NQ) |
| 3424 |
500.2302 | 998.4458 | 998.4345 | 11.4 | 1 | 2 | 2.5 | 1Score > 18 indicates identity |
QFNANFEK + 2 Deamidated (NQ) |
| 3523 |
373.5630 | 1117.6672 | 1117.6495 | 15.8 | 0 | 2 | 0.69 | 1Score > 13 indicates identity |
LIYGLGVINR + Deamidated (NQ) |
| 3653 |
635.8256 | 1269.6366 | 1269.6461 | -7.44 | 0 | 2 | 1 | 1Score > 20 indicates identity Score > 14 indicates homology |
MLSGYIAGAIMK + Oxidation (M) |
| 4398 |
1160.6090 | 3478.8052 | 3478.7654 | 11.4 | 1 | 2 | 1.3 | 1Score > 15 indicates identity |
ASIFCKATGLSTTISNGVTSSIDAIIYASDFLR |
| 3724 |
470.5610 | 1408.6612 | 1408.6888 | -19.6 | 1 | 2 | 1 | 1Score > 20 indicates identity Score > 14 indicates homology |
QWQQLFFQER |
| 3834 |
568.9721 | 1703.8945 | 1703.8763 | 10.6 | 0 | 2 | 2.6 | 1Score > 18 indicates identity |
SAIDLGIAMQLTNISR + 2 Deamidated (NQ) |
| 3713 |
462.5749 | 1384.7029 | 1384.7099 | -5.06 | 0 | 2 | 0.93 | 1Score > 20 indicates identity Score > 14 indicates homology |
VNLLGQNVNAWR + 2 Deamidated (NQ) |
| 3774 |
499.2677 | 1494.7813 | 1494.8082 | -18.0 | 0 | 2 | 3.4 | 1Score > 19 indicates identity |
VQPINTALIPNWK + 2 Deamidated (NQ) |
| 3790 |
774.9239 | 1547.8332 | 1547.8130 | 13.1 | 1 | 2 | 2.9 | 1Score > 19 indicates identity |
MNAPDIKALFSSVR |
| 3912 |
923.9672 | 1845.9198 | 1845.9472 | -14.8 | 1 | 2 | 1 | 1Score > 21 indicates identity Score > 14 indicates homology |
SESVQSNSAVLVHFTLK + Deamidated (NQ) |
| 4115 |
578.3135 | 2309.2249 | 2309.1903 | 15.0 | 1 | 2 | 2.3 | 1Score > 18 indicates identity |
VGDVIDVGPLHITIIEANDYR + Deamidated (NQ) |
| 4205 |
827.0917 | 2478.2533 | 2478.2179 | 14.3 | 0 | 1 | 2.7 | 1Score > 18 indicates identity |
QPVQFANTITHLHHAGVTTYLE + 2 Deamidated (NQ) |
| 3994 |
540.0541 | 2156.1873 | 2156.1987 | -5.28 | 1 | 1 | 0.93 | 1Score > 14 indicates identity |
DILQMQQLLSALIQRLTR + Deamidated (NQ); Oxidation (M) |
| 3524 |
373.5631 | 1117.6675 | 1117.6495 | 16.1 | 0 | 1 | 0.76 | 1Score > 13 indicates identity |
LIYGLGVINR + Deamidated (NQ) |
| 3641 |
630.8537 | 1259.6928 | 1259.6808 | 9.54 | 1 | 1 | 2.8 | 1Score > 18 indicates identity |
HYLKMLTQAR |
| 3525 |
559.8412 | 1117.6678 | 1117.6608 | 6.34 | 0 | 1 | 0.76 | 1Score > 13 indicates identity |
HANLPVLVVR + Deamidated (NQ) |
| 4090 |
457.4543 | 2282.2351 | 2282.2787 | -19.1 | 0 | 1 | 1.4 | 1Score > 15 indicates identity |
GWAIPAATDIAFALGVLALLGSR |
| 3468 |
525.7634 | 1049.5122 | 1049.5254 | -12.5 | 0 | 1 | 0.98 | 1Score > 21 indicates identity Score > 14 indicates homology |
QVLQHGDPR + Deamidated (NQ) |
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