MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2
MS data file : PRT1311_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:18:13 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,460

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1801–1900 (out of 4256)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1530 355.1929 354.1856
1531 355.1931 354.1858
1532 355.1932 354.1859
1533 355.1932 354.1859
1534 355.1935 354.1862
1535 355.2685 354.2612
1536 355.2686 354.2613
1537 356.1324 355.1251
1538 356.2721 355.2648
1539 356.2722 355.2649
1540 357.0995 356.0922
1541 357.1129 356.1056
1542 357.1145 356.1072
1543 357.1154 356.1081
1544 357.1199 356.1126
1545 357.1511 356.1438
1546 357.1552 356.1479
1547 357.1556 356.1483
1548 357.1705 356.1632
1549 357.1706 356.1633
1550 357.1911 356.1838
1551 359.1106 358.1033
1552 359.1112 358.1039
1553 359.1117 358.1044
1554 359.1117 358.1044
1555 359.1118 358.1045
1556 359.1125 358.1052
1557 359.1125 358.1052
1558 359.1479 358.1406
1559 359.1479 358.1406
1560 359.1482 358.1409
1561 359.1483 358.1410
1562 359.1486 358.1413
1563 359.1486 358.1413
1564 359.1486 358.1413
1565 359.1487 358.1414
1566 359.1487 358.1414
1567 359.1487 358.1414
1568 359.1488 358.1415
1569 359.1716 358.1643
1570 359.2419 358.2346
1571 359.2421 358.2348
1572 361.1270 360.1197
1573 361.1270 360.1197
1574 361.1273 360.1200
1575 361.1274 360.1201
1576 361.1277 360.1204
1577 361.1277 360.1204
1578 361.1641 360.1568
1579 361.1642 360.1569
1580 361.1643 360.1570
1581 361.1643 360.1570
1582 361.1644 360.1571
1583 361.1644 360.1571
1584 361.1645 360.1572
1585 361.1645 360.1572
1586 361.1645 360.1572
1587 361.1646 360.1573
1588 361.1646 360.1573
1589 361.1646 360.1573
1590 361.1646 360.1573
1591 361.1646 360.1573
1592 361.1646 360.1573
1593 361.1646 360.1573
1594 361.1646 360.1573
1595 361.1647 360.1574
1596 361.1847 360.1774
1597 361.2007 360.1934
1598 361.2008 360.1935
1599 361.2009 360.1936
1600 361.2010 360.1937
1601 361.2012 360.1939
1602 361.9282 360.9209
1603 361.9286 360.9213
1604 361.9397 360.9324
1605 362.0704 361.0631
1606 362.9988 361.9915
1607 363.1042 362.0969
1608 363.1134 362.1061
1609 363.1410 362.1337
1610 363.1423 362.1350
1611 363.1425 362.1352
1612 363.1425 362.1352
1613 363.1431 362.1358
1614 363.1432 362.1359
1615 363.1433 362.1360
1616 363.1435 362.1362
1617 363.1436 362.1363
1618 363.1436 362.1363
1619 363.1436 362.1363
1620 363.1436 362.1363
1621 363.1437 362.1364
1622 363.1437 362.1364
1623 363.1438 362.1365
1624 363.1438 362.1365
1625 363.1438 362.1365
1626 363.1798 362.1725
1627 363.1798 362.1725
1628 363.1799 362.1726
1629 363.1799 362.1726
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