MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2
MS data file : PRT1311_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:18:13 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,460

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1701–1800 (out of 4256)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1430 352.2201 351.2128
1431 352.2202 351.2129
1432 353.0434 352.0361
1433 353.0686 352.0613
1434 353.0709 352.0636
1435 353.0855 352.0782
1436 353.0856 352.0783
1437 353.1198 352.1125
1438 353.1206 352.1133
1439 353.1211 352.1138
1440 353.1220 352.1147
1441 353.1223 352.1150
1442 353.1223 352.1150
1443 353.1224 352.1151
1444 353.1225 352.1152
1445 353.1227 352.1154
1446 353.1230 352.1157
1447 353.1584 352.1511
1448 353.1587 352.1514
1449 353.1587 352.1514
1450 353.1591 352.1518
1451 353.1592 352.1519
1452 353.1593 352.1520
1453 353.1593 352.1520
1454 353.1595 352.1522
1455 353.1595 352.1522
1456 353.1595 352.1522
1457 353.1596 352.1523
1458 353.1597 352.1524
1459 353.1597 352.1524
1460 353.1597 352.1524
1461 353.1597 352.1524
1462 353.1598 352.1525
1463 353.1599 352.1526
1464 353.1599 352.1526
1465 353.1771 352.1698
1466 353.1771 352.1698
1467 353.1771 352.1698
1468 353.1772 352.1699
1469 353.1774 352.1701
1470 353.1957 352.1884
1471 353.1958 352.1885
1472 353.1961 352.1888
1473 353.1965 352.1892
1474 353.2146 352.2073
1475 353.2150 352.2077
1476 353.2526 352.2453
1477 353.2528 352.2455
1478 353.2534 352.2461
1479 353.2536 352.2463
1480 354.2558 353.2485
1481 354.9505 353.9432
1482 354.9507 353.9434
1483 355.0383 354.0310
1484 355.0389 354.0316
1485 355.0390 354.0317
1486 355.0708 354.0635
1487 355.0714 354.0641
1488 355.0714 354.0641
1489 355.0718 354.0645
1490 355.0718 354.0645
1491 355.0718 354.0645
1492 355.0719 354.0646
1493 355.0720 354.0647
1494 355.0721 354.0648
1495 355.0721 354.0648
1496 355.0722 354.0649
1497 355.0724 354.0651
1498 355.0725 354.0652
1499 355.0727 354.0654
1500 355.0825 354.0752
1501 355.0835 354.0762
1502 355.0837 354.0764
1503 355.0839 354.0766
1504 355.1079 354.1006
1505 355.1082 354.1009
1506 355.1088 354.1015
1507 355.1099 354.1026
1508 355.1357 354.1284
1509 355.1363 354.1290
1510 355.1364 354.1291
1511 355.1374 354.1301
1512 355.1381 354.1308
1513 355.1383 354.1310
1514 355.1386 354.1313
1515 355.1388 354.1315
1516 355.1392 354.1319
1517 355.1642 354.1569
1518 355.1645 354.1572
1519 355.1750 354.1677
1520 355.1752 354.1679
1521 355.1754 354.1681
1522 355.1761 354.1688
1523 355.1763 354.1690
1524 355.1764 354.1691
1525 355.1765 354.1692
1526 355.1766 354.1693
1527 355.1767 354.1694
1528 355.1923 354.1850
1529 355.1928 354.1855
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