MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2
MS data file : PRT1311_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:18:13 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,460

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1601–1700 (out of 4256)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1330 349.2006 348.1933
1331 349.2006 348.1933
1332 349.2006 348.1933
1333 349.2006 348.1933
1334 349.2006 348.1933
1335 349.2007 348.1934
1336 349.2007 348.1934
1337 349.2008 348.1935
1338 349.2008 348.1935
1339 349.2008 348.1935
1340 349.2008 348.1935
1341 349.2011 348.1938
1342 349.2016 348.1943
1343 349.2205 348.2132
1344 349.2208 348.2135
1345 349.2208 348.2135
1346 349.2211 348.2138
1347 349.2212 348.2139
1348 349.2212 348.2139
1349 349.2213 348.2140
1350 349.2214 348.2141
1351 349.2217 348.2144
1352 349.2223 348.2150
1353 349.2227 348.2154
1354 349.9836 348.9763
1355 350.2309 349.2236
1356 350.9779 349.9706
1357 351.0490 350.0417
1358 351.0775 350.0702
1359 351.0908 350.0835
1360 351.1049 350.0976
1361 351.1403 350.1330
1362 351.1416 350.1343
1363 351.1423 350.1350
1364 351.1426 350.1353
1365 351.1429 350.1356
1366 351.1433 350.1360
1367 351.1435 350.1362
1368 351.1435 350.1362
1369 351.1436 350.1363
1370 351.1436 350.1363
1371 351.1437 350.1364
1372 351.1437 350.1364
1373 351.1437 350.1364
1374 351.1438 350.1365
1375 351.1789 350.1716
1376 351.1794 350.1721
1377 351.1795 350.1722
1378 351.1798 350.1725
1379 351.1798 350.1725
1380 351.1799 350.1726
1381 351.1799 350.1726
1382 351.1800 350.1727
1383 351.1800 350.1727
1384 351.1800 350.1727
1385 351.1800 350.1727
1386 351.1801 350.1728
1387 351.1801 350.1728
1388 351.1801 350.1728
1389 351.1801 350.1728
1390 351.1801 350.1728
1391 351.1801 350.1728
1392 351.1802 350.1729
1393 351.1805 350.1732
1394 351.1977 350.1904
1395 351.1981 350.1908
1396 351.1983 350.1910
1397 351.1985 350.1912
1398 351.1988 350.1915
1399 351.2161 350.2088
1400 351.2162 350.2089
1401 351.2162 350.2089
1402 351.2162 350.2089
1403 351.2162 350.2089
1404 351.2163 350.2090
1405 351.2163 350.2090
1406 351.2163 350.2090
1407 351.2163 350.2090
1408 351.2164 350.2091
1409 351.2164 350.2091
1410 351.2165 350.2092
1411 351.2166 350.2093
1412 351.2172 350.2099
1413 351.2366 350.2293
1414 351.2366 350.2293
1415 351.2368 350.2295
1416 351.2368 350.2295
1417 351.2369 350.2296
1418 351.2370 350.2297
1419 351.2370 350.2297
1420 351.2370 350.2297
1421 351.2371 350.2298
1422 351.2372 350.2299
1423 351.2372 350.2299
1424 351.2378 350.2305
1425 351.2379 350.2306
1426 352.0121 351.0048
1427 352.0123 351.0050
1428 352.2197 351.2124
1429 352.2197 351.2124
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