MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2
MS data file : PRT1311_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:18:13 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,460

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1501–1600 (out of 4256)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1230 345.1696 344.1623
1231 345.1697 344.1624
1232 345.1699 344.1626
1233 345.1700 344.1627
1234 345.1700 344.1627
1235 345.1700 344.1627
1236 345.2262 344.2189
1237 345.2262 344.2189
1238 345.2264 344.2191
1239 345.2266 344.2193
1240 345.2267 344.2194
1241 345.2775 344.2702
1242 346.0371 345.0298
1243 346.9007 345.8934
1244 346.9008 345.8935
1245 346.9009 345.8936
1246 347.1107 346.1034
1247 347.1110 346.1037
1248 347.1118 346.1045
1249 347.1478 346.1405
1250 347.1482 346.1409
1251 347.1483 346.1410
1252 347.1483 346.1410
1253 347.1483 346.1410
1254 347.1484 346.1411
1255 347.1484 346.1411
1256 347.1485 346.1412
1257 347.1485 346.1412
1258 347.1485 346.1412
1259 347.1485 346.1412
1260 347.1485 346.1412
1261 347.1486 346.1413
1262 347.1487 346.1414
1263 347.1487 346.1414
1264 347.1487 346.1414
1265 347.1488 346.1415
1266 347.1848 346.1775
1267 347.1848 346.1775
1268 347.1848 346.1775
1269 347.1848 346.1775
1270 347.1848 346.1775
1271 347.1849 346.1776
1272 347.1849 346.1776
1273 347.1849 346.1776
1274 347.1849 346.1776
1275 347.1850 346.1777
1276 347.1850 346.1777
1277 347.1850 346.1777
1278 347.1850 346.1777
1279 347.1851 346.1778
1280 347.1851 346.1778
1281 347.1852 346.1779
1282 347.1852 346.1779
1283 347.1852 346.1779
1284 347.1855 346.1782
1285 347.1857 346.1784
1286 347.2061 346.1988
1287 348.0525 347.0452
1288 348.0531 347.0458
1289 349.1254 348.1181
1290 349.1257 348.1184
1291 349.1262 348.1189
1292 349.1267 348.1194
1293 349.1274 348.1201
1294 349.1275 348.1202
1295 349.1275 348.1202
1296 349.1276 348.1203
1297 349.1276 348.1203
1298 349.1276 348.1203
1299 349.1276 348.1203
1300 349.1277 348.1204
1301 349.1277 348.1204
1302 349.1277 348.1204
1303 349.1278 348.1205
1304 349.1279 348.1206
1305 349.1635 348.1562
1306 349.1636 348.1563
1307 349.1639 348.1566
1308 349.1640 348.1567
1309 349.1640 348.1567
1310 349.1640 348.1567
1311 349.1641 348.1568
1312 349.1641 348.1568
1313 349.1642 348.1569
1314 349.1642 348.1569
1315 349.1642 348.1569
1316 349.1642 348.1569
1317 349.1642 348.1569
1318 349.1643 348.1570
1319 349.1643 348.1570
1320 349.1645 348.1572
1321 349.1645 348.1572
1322 349.1645 348.1572
1323 349.1645 348.1572
1324 349.1645 348.1572
1325 349.1647 348.1574
1326 349.1647 348.1574
1327 349.1855 348.1782
1328 349.2005 348.1932
1329 349.2006 348.1933
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