MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2
MS data file : PRT1311_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:18:13 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,460

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1401–1500 (out of 4256)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1130 339.1995 338.1922
1131 339.1995 338.1922
1132 339.1996 338.1923
1133 339.1999 338.1926
1134 339.2006 338.1933
1135 340.1643 339.1570
1136 340.2607 339.2534
1137 340.2615 339.2542
1138 340.9343 339.9270
1139 341.0228 340.0155
1140 341.0914 340.0841
1141 341.1185 340.1112
1142 341.1186 340.1113
1143 341.1195 340.1122
1144 341.1196 340.1123
1145 341.1206 340.1133
1146 341.1211 340.1138
1147 341.1379 340.1306
1148 341.1381 340.1308
1149 341.1384 340.1311
1150 341.1385 340.1312
1151 341.1388 340.1315
1152 341.1469 340.1396
1153 341.1475 340.1402
1154 341.1475 340.1402
1155 341.1479 340.1406
1156 341.1484 340.1411
1157 341.1486 340.1413
1158 341.1589 340.1516
1159 341.1589 340.1516
1160 341.1591 340.1518
1161 341.1592 340.1519
1162 341.1593 340.1520
1163 341.1595 340.1522
1164 341.1595 340.1522
1165 341.1595 340.1522
1166 341.1596 340.1523
1167 341.1597 340.1524
1168 341.1598 340.1525
1169 341.1599 340.1526
1170 341.1602 340.1529
1171 341.1960 340.1887
1172 341.1963 340.1890
1173 341.1964 340.1891
1174 341.1967 340.1894
1175 341.1968 340.1895
1176 341.1969 340.1896
1177 341.1969 340.1896
1178 341.1971 340.1898
1179 341.1971 340.1898
1180 341.1972 340.1899
1181 341.1973 340.1900
1182 341.1973 340.1900
1183 341.1974 340.1901
1184 341.1974 340.1901
1185 341.1975 340.1902
1186 341.1975 340.1902
1187 341.2161 340.2088
1188 341.2162 340.2089
1189 341.2162 340.2089
1190 341.2164 340.2091
1191 342.0414 341.0341
1192 342.1207 341.1134
1193 343.0354 342.0281
1194 343.0973 342.0900
1195 343.0975 342.0902
1196 343.0982 342.0909
1197 343.0986 342.0913
1198 343.0989 342.0916
1199 343.0994 342.0921
1200 343.1154 342.1081
1201 343.1159 342.1086
1202 343.1159 342.1086
1203 343.1166 342.1093
1204 343.1347 342.1274
1205 343.1535 342.1462
1206 343.1536 342.1463
1207 343.1538 342.1465
1208 343.1541 342.1468
1209 343.1768 342.1695
1210 343.2321 342.2248
1211 343.2982 342.2909
1212 345.1128 344.1055
1213 345.1321 344.1248
1214 345.1324 344.1251
1215 345.1324 344.1251
1216 345.1325 344.1252
1217 345.1325 344.1252
1218 345.1326 344.1253
1219 345.1327 344.1254
1220 345.1328 344.1255
1221 345.1330 344.1257
1222 345.1330 344.1257
1223 345.1332 344.1259
1224 345.1340 344.1267
1225 345.1687 344.1614
1226 345.1693 344.1620
1227 345.1694 344.1621
1228 345.1694 344.1621
1229 345.1695 344.1622
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