MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2
MS data file : PRT1311_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:18:13 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,460

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1301–1400 (out of 4256)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1030 337.1273 336.1200
1031 337.1276 336.1203
1032 337.1276 336.1203
1033 337.1277 336.1204
1034 337.1277 336.1204
1035 337.1277 336.1204
1036 337.1278 336.1205
1037 337.1278 336.1205
1038 337.1279 336.1206
1039 337.1281 336.1208
1040 337.1291 336.1218
1041 337.1295 336.1222
1042 337.1641 336.1568
1043 337.1641 336.1568
1044 337.1641 336.1568
1045 337.1642 336.1569
1046 337.1642 336.1569
1047 337.1642 336.1569
1048 337.1642 336.1569
1049 337.1642 336.1569
1050 337.1643 336.1570
1051 337.1643 336.1570
1052 337.1644 336.1571
1053 337.1644 336.1571
1054 337.1645 336.1572
1055 337.1645 336.1572
1056 337.1645 336.1572
1057 337.1646 336.1573
1058 337.1646 336.1573
1059 337.1646 336.1573
1060 337.1648 336.1575
1061 337.1818 336.1745
1062 337.1820 336.1747
1063 337.1822 336.1749
1064 337.1822 336.1749
1065 337.1823 336.1750
1066 337.1823 336.1750
1067 337.1824 336.1751
1068 337.1826 336.1753
1069 337.1826 336.1753
1070 337.1827 336.1754
1071 337.1829 336.1756
1072 337.2006 336.1933
1073 337.2008 336.1935
1074 337.2010 336.1937
1075 337.2368 336.2295
1076 337.2575 336.2502
1077 337.2577 336.2504
1078 337.2581 336.2508
1079 337.2582 336.2509
1080 338.0560 337.0487
1081 338.0568 337.0495
1082 338.1653 337.1580
1083 338.1656 337.1583
1084 338.1657 337.1584
1085 338.1657 337.1584
1086 338.1658 337.1585
1087 338.1660 337.1587
1088 338.1660 337.1587
1089 339.0692 338.0619
1090 339.1031 338.0958
1091 339.1035 338.0962
1092 339.1044 338.0971
1093 339.1053 338.0980
1094 339.1062 338.0989
1095 339.1062 338.0989
1096 339.1082 338.1009
1097 339.1128 338.1055
1098 339.1130 338.1057
1099 339.1132 338.1059
1100 339.1413 338.1340
1101 339.1415 338.1342
1102 339.1426 338.1353
1103 339.1428 338.1355
1104 339.1431 338.1358
1105 339.1433 338.1360
1106 339.1435 338.1362
1107 339.1435 338.1362
1108 339.1436 338.1363
1109 339.1436 338.1363
1110 339.1436 338.1363
1111 339.1437 338.1364
1112 339.1437 338.1364
1113 339.1437 338.1364
1114 339.1438 338.1365
1115 339.1493 338.1420
1116 339.1494 338.1421
1117 339.1499 338.1426
1118 339.1604 338.1531
1119 339.1795 338.1722
1120 339.1795 338.1722
1121 339.1801 338.1728
1122 339.1802 338.1729
1123 339.1982 338.1909
1124 339.1986 338.1913
1125 339.1988 338.1915
1126 339.1989 338.1916
1127 339.1990 338.1917
1128 339.1992 338.1919
1129 339.1994 338.1921
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