MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2
MS data file : PRT1311_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:18:13 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,460

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1201–1300 (out of 4256)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
930 333.2055 332.1982
931 333.2055 332.1982
932 333.2055 332.1982
933 333.2055 332.1982
934 333.2055 332.1982
935 333.2056 332.1983
936 333.2056 332.1983
937 333.2058 332.1985
938 333.2065 332.1992
939 333.2242 332.2169
940 333.2244 332.2171
941 333.2249 332.2176
942 333.2253 332.2180
943 333.2254 332.2181
944 333.2255 332.2182
945 333.2255 332.2182
946 333.2259 332.2186
947 333.2285 332.2212
948 334.0110 333.0037
949 334.0111 333.0038
950 334.1345 333.1272
951 335.0321 334.0248
952 335.0324 334.0251
953 335.0326 334.0253
954 335.1084 334.1011
955 335.1103 334.1030
956 335.1104 334.1031
957 335.1107 334.1034
958 335.1113 334.1040
959 335.1114 334.1041
960 335.1114 334.1041
961 335.1114 334.1041
962 335.1118 334.1045
963 335.1482 334.1409
964 335.1483 334.1410
965 335.1484 334.1411
966 335.1484 334.1411
967 335.1485 334.1412
968 335.1485 334.1412
969 335.1486 334.1413
970 335.1486 334.1413
971 335.1486 334.1413
972 335.1486 334.1413
973 335.1487 334.1414
974 335.1487 334.1414
975 335.1487 334.1414
976 335.1487 334.1414
977 335.1488 334.1415
978 335.1490 334.1417
979 335.1846 334.1773
980 335.1846 334.1773
981 335.1847 334.1774
982 335.1847 334.1774
983 335.1848 334.1775
984 335.1848 334.1775
985 335.1848 334.1775
986 335.1849 334.1776
987 335.1849 334.1776
988 335.1850 334.1777
989 335.1850 334.1777
990 335.1850 334.1777
991 335.1851 334.1778
992 335.1851 334.1778
993 335.1851 334.1778
994 335.1852 334.1779
995 335.1854 334.1781
996 335.1856 334.1783
997 335.1859 334.1786
998 335.2210 334.2137
999 335.2211 334.2138
1000 335.2211 334.2138
1001 335.2211 334.2138
1002 335.2212 334.2139
1003 335.2212 334.2139
1004 335.2212 334.2139
1005 335.2212 334.2139
1006 335.2213 334.2140
1007 335.2213 334.2140
1008 335.2213 334.2140
1009 335.2213 334.2140
1010 335.2216 334.2143
1011 335.2222 334.2149
1012 335.2407 334.2334
1013 335.2408 334.2335
1014 335.2409 334.2336
1015 335.2409 334.2336
1016 335.2415 334.2342
1017 335.2422 334.2349
1018 335.9286 334.9213
1019 335.9677 334.9604
1020 335.9678 334.9605
1021 335.9679 334.9606
1022 335.9679 334.9606
1023 336.0162 335.0089
1024 336.1887 335.1814
1025 337.0725 336.0652
1026 337.1244 336.1171
1027 337.1247 336.1174
1028 337.1256 336.1183
1029 337.1271 336.1198
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