MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2
MS data file : PRT1311_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:18:13 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,460

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1101–1200 (out of 4256)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
830 329.1371 328.1298
831 329.1371 328.1298
832 329.1374 328.1301
833 329.1376 328.1303
834 329.1378 328.1305
835 329.1379 328.1306
836 329.1379 328.1306
837 329.1379 328.1306
838 329.1380 328.1307
839 329.1595 328.1522
840 329.1598 328.1525
841 329.1599 328.1526
842 329.1600 328.1527
843 329.1602 328.1529
844 329.1603 328.1530
845 329.1742 328.1669
846 329.2167 328.2094
847 330.0487 329.0414
848 331.0978 330.0905
849 331.1158 330.1085
850 331.1162 330.1089
851 331.1164 330.1091
852 331.1165 330.1092
853 331.1166 330.1093
854 331.1169 330.1096
855 331.1169 330.1096
856 331.1326 330.1253
857 331.1528 330.1455
858 331.1529 330.1456
859 331.1529 330.1456
860 331.1530 330.1457
861 331.1531 330.1458
862 331.1533 330.1460
863 331.1534 330.1461
864 331.1534 330.1461
865 331.1535 330.1462
866 331.1535 330.1462
867 331.1535 330.1462
868 331.1536 330.1463
869 331.1537 330.1464
870 331.1538 330.1465
871 331.1539 330.1466
872 331.1767 330.1694
873 331.1772 330.1699
874 331.1896 330.1823
875 331.1899 330.1826
876 331.1899 330.1826
877 331.1899 330.1826
878 331.1900 330.1827
879 331.1901 330.1828
880 331.1902 330.1829
881 331.1905 330.1832
882 331.1906 330.1833
883 331.2103 330.2030
884 331.2104 330.2031
885 333.0559 332.0486
886 333.0561 332.0488
887 333.0565 332.0492
888 333.0566 332.0493
889 333.1320 332.1247
890 333.1321 332.1248
891 333.1322 332.1249
892 333.1323 332.1250
893 333.1324 332.1251
894 333.1325 332.1252
895 333.1327 332.1254
896 333.1328 332.1255
897 333.1329 332.1256
898 333.1331 332.1258
899 333.1346 332.1273
900 333.1487 332.1414
901 333.1488 332.1415
902 333.1490 332.1417
903 333.1684 332.1611
904 333.1688 332.1615
905 333.1688 332.1615
906 333.1689 332.1616
907 333.1690 332.1617
908 333.1690 332.1617
909 333.1691 332.1618
910 333.1691 332.1618
911 333.1691 332.1618
912 333.1691 332.1618
913 333.1692 332.1619
914 333.1692 332.1619
915 333.1692 332.1619
916 333.1692 332.1619
917 333.1692 332.1619
918 333.1692 332.1619
919 333.1693 332.1620
920 333.1694 332.1621
921 333.1695 332.1622
922 333.1698 332.1625
923 333.1699 332.1626
924 333.1903 332.1830
925 333.2054 332.1981
926 333.2054 332.1981
927 333.2054 332.1981
928 333.2054 332.1981
929 333.2055 332.1982
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