MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2
MS data file : PRT1311_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:18:13 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,460

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1001–1100 (out of 4256)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
730 325.1645 324.1572
731 325.1646 324.1573
732 325.1646 324.1573
733 325.1647 324.1574
734 325.1647 324.1574
735 325.1647 324.1574
736 325.1647 324.1574
737 325.1647 324.1574
738 325.1649 324.1576
739 325.1649 324.1576
740 325.1654 324.1581
741 325.1820 324.1747
742 325.1820 324.1747
743 325.1823 324.1750
744 325.1824 324.1751
745 325.1825 324.1752
746 325.1827 324.1754
747 325.1831 324.1758
748 325.1833 324.1760
749 325.2013 324.1940
750 325.2017 324.1944
751 325.2017 324.1944
752 325.2018 324.1945
753 325.2018 324.1945
754 325.2018 324.1945
755 325.2019 324.1946
756 325.2019 324.1946
757 325.2021 324.1948
758 325.2021 324.1948
759 325.2022 324.1949
760 325.2022 324.1949
761 325.2209 324.2136
762 325.2210 324.2137
763 325.2212 324.2139
764 325.2213 324.2140
765 325.9389 324.9316
766 326.0126 325.0053
767 326.0899 325.0826
768 326.1477 325.1404
769 326.1477 325.1404
770 326.1478 325.1405
771 326.1482 325.1409
772 326.1485 325.1412
773 326.9788 325.9715
774 327.0525 326.0452
775 327.0539 326.0466
776 327.1021 326.0948
777 327.1036 326.0963
778 327.1048 326.0975
779 327.1052 326.0979
780 327.1055 326.0982
781 327.1214 326.1141
782 327.1215 326.1142
783 327.1216 326.1143
784 327.1222 326.1149
785 327.1224 326.1151
786 327.1317 326.1244
787 327.1317 326.1244
788 327.1319 326.1246
789 327.1320 326.1247
790 327.1320 326.1247
791 327.1320 326.1247
792 327.1329 326.1256
793 327.1430 326.1357
794 327.1437 326.1364
795 327.1440 326.1367
796 327.1441 326.1368
797 327.1443 326.1370
798 327.1446 326.1373
799 327.1446 326.1373
800 327.1454 326.1381
801 327.1456 326.1383
802 327.1797 326.1724
803 327.1799 326.1726
804 327.1801 326.1728
805 327.1808 326.1735
806 327.1808 326.1735
807 327.1813 326.1740
808 327.1813 326.1740
809 327.1813 326.1740
810 327.1998 326.1925
811 327.2000 326.1927
812 327.2003 326.1930
813 327.2003 326.1930
814 327.2003 326.1930
815 327.2004 326.1931
816 327.2004 326.1931
817 327.2033 326.1960
818 327.2374 326.2301
819 328.0172 327.0099
820 328.0177 327.0104
821 328.1042 327.0969
822 328.1045 327.0972
823 328.1047 327.0974
824 329.0673 328.0600
825 329.0822 328.0749
826 329.0824 328.0751
827 329.0826 328.0753
828 329.1118 328.1045
829 329.1190 328.1117
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