MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2
MS data file : PRT1311_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:18:13 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,460

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 901–1000 (out of 4256)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
630 321.2618 320.2545
631 321.9751 320.9678
632 322.2455 321.2382
633 322.9827 321.9754
634 322.9829 321.9756
635 322.9830 321.9757
636 322.9830 321.9757
637 322.9834 321.9761
638 322.9835 321.9762
639 322.9835 321.9762
640 322.9836 321.9763
641 322.9837 321.9764
642 322.9837 321.9764
643 323.0714 322.0641
644 323.1092 322.1019
645 323.1095 322.1022
646 323.1096 322.1023
647 323.1102 322.1029
648 323.1106 322.1033
649 323.1108 322.1035
650 323.1109 322.1036
651 323.1109 322.1036
652 323.1110 322.1037
653 323.1115 322.1042
654 323.1116 322.1043
655 323.1116 322.1043
656 323.1468 322.1395
657 323.1476 322.1403
658 323.1477 322.1404
659 323.1479 322.1406
660 323.1480 322.1407
661 323.1481 322.1408
662 323.1481 322.1408
663 323.1483 322.1410
664 323.1484 322.1411
665 323.1484 322.1411
666 323.1485 322.1412
667 323.1485 322.1412
668 323.1486 322.1413
669 323.1486 322.1413
670 323.1487 322.1414
671 323.1487 322.1414
672 323.1488 322.1415
673 323.1660 322.1587
674 323.1661 322.1588
675 323.1665 322.1592
676 323.1847 322.1774
677 323.1849 322.1776
678 323.1850 322.1777
679 323.1851 322.1778
680 323.1852 322.1779
681 323.1852 322.1779
682 323.1853 322.1780
683 323.1853 322.1780
684 323.1853 322.1780
685 323.1855 322.1782
686 323.1855 322.1782
687 323.1855 322.1782
688 323.1856 322.1783
689 323.1856 322.1783
690 323.1856 322.1783
691 323.1857 322.1784
692 323.1857 322.1784
693 323.1857 322.1784
694 323.1858 322.1785
695 323.1859 322.1786
696 323.2046 322.1973
697 323.2047 322.1974
698 323.2047 322.1974
699 323.2047 322.1974
700 323.2048 322.1975
701 323.2202 322.2129
702 323.9665 322.9592
703 323.9673 322.9600
704 324.0171 323.0098
705 324.1502 323.1429
706 324.1518 323.1445
707 324.1520 323.1447
708 324.9836 323.9763
709 325.0870 324.0797
710 325.0871 324.0798
711 325.0885 324.0812
712 325.0890 324.0817
713 325.0892 324.0819
714 325.0894 324.0821
715 325.1264 324.1191
716 325.1264 324.1191
717 325.1270 324.1197
718 325.1270 324.1197
719 325.1272 324.1199
720 325.1272 324.1199
721 325.1276 324.1203
722 325.1277 324.1204
723 325.1277 324.1204
724 325.1280 324.1207
725 325.1280 324.1207
726 325.1280 324.1207
727 325.1333 324.1260
728 325.1340 324.1267
729 325.1341 324.1268
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