MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Andy2 |
| MS data file | : | PRT1311_JBEI_2.mgf |
| Database | : | Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues) |
| Timestamp | : | 8 Sep 2025 at 17:18:13 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,460 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| Score | Mass | Matches | Sequences | emPAI | |||
| 1 | Q88JG6_PSEPK | 2626 | 61597 | 122 (98) | 25 (22) | 2.45 | histidine kinase OS=Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) OX=160488 GN=PP_2683 PE=4 SV=2 |
| 2 | TRYP_PIG | 225 | 25078 | 9 (7) | 3 (3) | 0.30 | Trypsin OS=Sus scrofa PE=1 SV=1 |
| 3 | Q70I01_9ACTO | 193 | 166730 | 15 (10) | 7 (5) | 0.13 | Borrelidin polyketide synthase, type I OS=Streptomyces parvulus GN=borA2 PE=4 SV=1 |
| 4 | CH60_ECOLI | 102 | 57464 | 20 (13) | 17 (11) | 0.60 | 60 kDa chaperonin OS=Escherichia coli (strain K12) GN=groL PE=1 SV=2 |
| 5 | Q88JG7_PSEPK | 42 | 44735 | 7 (4) | 6 (4) | 0.22 | Fe-containing alcohol dehydrogenase OS=Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) OX=160488 GN=yiaY PE=3 SV=1 |
| 6 | K1C10_HUMAN | 31 | 59020 | 4 (1) | 4 (1) | 0.04 | Keratin, type I cytoskeletal 10 OS=Homo sapiens GN=KRT10 PE=1 SV=6 |
| 7 | GLND_ECOLI | 31 | 102839 | 1 (1) | 1 (1) | 0.02 | Bifunctional uridylyltransferase/uridylyl-removing enzyme OS=Escherichia coli (strain K12) GN=glnD PE=1 SV=2 |
| 8 | EFTU1_ECOLI | 30 | 43427 | 5 (2) | 4 (2) | 0.11 | Elongation factor Tu 1 OS=Escherichia coli (strain K12) GN=tufA PE=1 SV=1 |
| 9 | KPYK2_ECOLI | 29 | 51553 | 10 (2) | 10 (2) | 0.09 | Pyruvate kinase II OS=Escherichia coli (strain K12) GN=pykA PE=1 SV=3 |
| 10 | MSCM_ECOLI | 27 | 124232 | 1 (1) | 1 (1) | 0.02 | Miniconductance mechanosensitive channel MscM OS=Escherichia coli (strain K12) GN=mscM PE=1 SV=3 |
| 11 | UGPC_ECOLI | 25 | 39556 | 2 (1) | 2 (1) | 0.06 | sn-glycerol-3-phosphate import ATP-binding protein UgpC OS=Escherichia coli (strain K12) GN=ugpC PE=1 SV=3 |
| 12 | WECD_ECOLI | 25 | 24262 | 1 (1) | 1 (1) | 0.10 | dTDP-fucosamine acetyltransferase OS=Escherichia coli (strain K12) GN=wecD PE=3 SV=3 |
| 13 | TGT_ECOLI | 25 | 0 | 1 (1) | 1 (1) | 0.06 | description |
| 14 | A4XUL5_PSEMY | 23 | 79167 | 3 (1) | 2 (1) | 0.03 | Catalase OS=Pseudomonas mendocina (strain ymp) GN=Pmen_2273 PE=3 SV=1 |
| 15 | GLTB_ECOLI | 22 | 164335 | 1 (1) | 1 (1) | 0.01 | Glutamate synthase [NADPH] large chain OS=Escherichia coli (strain K12) GN=gltB PE=1 SV=3 |
| 16 | GLPD_ECOLI | 21 | 56886 | 1 (1) | 1 (1) | 0.04 | Aerobic glycerol-3-phosphate dehydrogenase OS=Escherichia coli (strain K12) GN=glpD PE=1 SV=3 |
| 17 | NIKA_ECOLI | 21 | 0 | 1 (1) | 1 (1) | 0.04 | description |
| 18 | OPGG_ECOLI | 21 | 57876 | 3 (1) | 2 (1) | 0.04 | Glucans biosynthesis protein G OS=Escherichia coli (strain K12) GN=mdoG PE=1 SV=1 |
| 19 | FTSW_ECOLI | 15 | 46071 | 5 (1) | 1 (1) | 0.05 | Lipid II flippase FtsW OS=Escherichia coli (strain K12) GN=ftsW PE=1 SV=1 |
| 20 | GLTP_ECOLI | 14 | 0 | 2 (1) | 2 (1) | 0.05 | description |
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