MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Andy2 |
| MS data file | : | PRT1311_JBEI_2.mgf |
| Database | : | Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues) |
| Timestamp | : | 8 Sep 2025 at 17:18:13 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,460 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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1| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88JG6_PSEPK | 2626 | histidine kinase OS=Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) OX=160488 GN=PP_2683 PE=4 SV=2 |
2| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TRYP_PIG | 225 | Trypsin OS=Sus scrofa PE=1 SV=1 |
3| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q70I01_9ACTO | 193 | Borrelidin polyketide synthase, type I OS=Streptomyces parvulus GN=borA2 PE=4 SV=1 |
4| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CH60_ECOLI | 102 | 60 kDa chaperonin OS=Escherichia coli (strain K12) GN=groL PE=1 SV=2 |
5| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88JG7_PSEPK | 42 | Fe-containing alcohol dehydrogenase OS=Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) OX=160488 GN=yiaY PE=3 SV=1 |
6| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | K1C10_HUMAN | 31 | Keratin, type I cytoskeletal 10 OS=Homo sapiens GN=KRT10 PE=1 SV=6 |
7| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | GLND_ECOLI | 31 | Bifunctional uridylyltransferase/uridylyl-removing enzyme OS=Escherichia coli (strain K12) GN=glnD PE=1 SV=2 |
8| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EFTU1_ECOLI | 30 | Elongation factor Tu 1 OS=Escherichia coli (strain K12) GN=tufA PE=1 SV=1 |
9| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | KPYK2_ECOLI | 29 | Pyruvate kinase II OS=Escherichia coli (strain K12) GN=pykA PE=1 SV=3 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 9.1 | KPYK2_ECOLI |
29 | 51553 | 10 (2) | 10 (2) | 0.09 | |
| Pyruvate kinase II OS=Escherichia coli (strain K12) GN=pykA PE=1 SV=3 | |||||||
10 peptide matches (10 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 3221 |
347.6945 | 693.3744 | 693.3697 | 6.80 | 0 | 2 | 3.2 | 1Score > 19 indicates identity |
U | K.VGIDYK.G | |
| 3318 |
429.2602 | 856.5058 | 856.5018 | 4.73 | 0 | 14 | 0.2 | 1Score > 19 indicates identity |
U | K.IPSINVSK.H | |
| 3346 |
460.2527 | 918.4908 | 918.4845 | 6.96 | 1 | 8 | 0.83 | 1Score > 20 indicates identity |
U | K.VLEVQGMK.V + Oxidation (M) | |
| 3380 |
485.2988 | 968.5830 | 968.5767 | 6.55 | 0 | 22 | 0.013 | 1Score > 16 indicates identity |
U | K.VIAAGANVVR.M | |
| 3479 |
532.8118 | 1063.6090 | 1063.6026 | 6.09 | 0 | 16 | 0.06 | 1Score > 17 indicates identity |
U | R.TLNLTALYR.G | |
| 3673 |
647.8485 | 1293.6824 | 1293.6751 | 5.68 | 0 | 10 | 0.41 | 1Score > 19 indicates identity |
U | R.ISSGLPIFAMSR.H + Oxidation (M) | |
D:\Xcalibur\Data\Jennifer\PRT1311 Andy\PRT1311_JBEI_2.rawScore > 20 indicates identity |
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| 3698 |
676.3521 | 1350.6896 | 1350.6813 | 6.17 | 1 | 25 | 0.017 | 1Score > 20 indicates identity |
U | K.GVTAIITMTESGR.T + Oxidation (M) | |
| No other peptide matches in query | |||||||||||
| 3792 |
781.4205 | 1560.8264 | 1560.8148 | 7.47 | 1 | 4 | 1 | 1Score > 20 indicates identity Score > 17 indicates homology |
U | K.VFTEVTVGGPLSNNK.G | |
| 4113 |
768.7750 | 2303.3032 | 2303.2737 | 12.8 | 1 | 2 | 0.66 | 1Score > 13 indicates identity |
U | K.GLPADVVPGDILLLDDGRVQLK.V + Deamidated (NQ) | |
| 4223 |
837.4292 | 2509.2658 | 2509.2561 | 3.85 | 1 | 16 | 0.089 | 1Score > 18 indicates identity |
U | R.GVTPVHFDSANDGVAAASEAVNLLR.D | |
10| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | MSCM_ECOLI | 27 | Miniconductance mechanosensitive channel MscM OS=Escherichia coli (strain K12) GN=mscM PE=1 SV=3 |
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