MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2
MS data file : PRT1311_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:18:13 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,460

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 20)


Page: 1 2 Next 

+1

Accession Score Description
1 Q88JG6_PSEPK 2626 histidine kinase OS=Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) OX=160488 GN=PP_2683 PE=4 SV=2

+2

Accession Score Description
1 TRYP_PIG 225 Trypsin OS=Sus scrofa PE=1 SV=1

+3

Accession Score Description
1 Q70I01_9ACTO 193 Borrelidin polyketide synthase, type I OS=Streptomyces parvulus GN=borA2 PE=4 SV=1

+4

Accession Score Description
1 CH60_ECOLI 102 60 kDa chaperonin OS=Escherichia coli (strain K12) GN=groL PE=1 SV=2

+5

Accession Score Description
1 Q88JG7_PSEPK 42 Fe-containing alcohol dehydrogenase OS=Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) OX=160488 GN=yiaY PE=3 SV=1

-6

Accession Score Description
1 K1C10_HUMAN 31 Keratin, type I cytoskeletal 10 OS=Homo sapiens GN=KRT10 PE=1 SV=6
Score Mass Matches Sequences emPAI
6.1 K1C10_HUMAN 31 59020 4 (1) 4 (1) 0.04
Keratin, type I cytoskeletal 10 OS=Homo sapiens GN=KRT10 PE=1 SV=6
9 samesets of K1C10_HUMAN
KRT36_HUMAN 31 53354 2 (1) 2 (1) 0.04
Keratin, type I cuticular Ha6 OS=Homo sapiens GN=KRT36 PE=1 SV=1
K1C15_SHEEP 31 48740 1 (1) 1 (1) 0.05
Keratin, type I cytoskeletal 15 OS=Ovis aries GN=KRT15 PE=2 SV=1
K1H1_HUMAN 31 48633 1 (1) 1 (1) 0.05
Keratin, type I cuticular Ha1 OS=Homo sapiens GN=KRT31 PE=2 SV=3
K1H2_HUMAN 31 51793 1 (1) 1 (1) 0.04
Keratin, type I cuticular Ha2 OS=Homo sapiens GN=KRT32 PE=1 SV=3
K1M1_SHEEP 31 48070 1 (1) 1 (1) 0.05
Keratin, type I microfibrillar 48 kDa, component 8C-1 OS=Ovis aries PE=1 SV=2
KRT35_HUMAN 31 51640 1 (1) 1 (1) 0.04
Keratin, type I cuticular Ha5 OS=Homo sapiens GN=KRT35 PE=2 SV=5
KRT37_HUMAN 31 51084 1 (1) 1 (1) 0.04
Keratin, type I cuticular Ha7 OS=Homo sapiens GN=KRT37 PE=2 SV=3
KRT38_HUMAN 31 52044 1 (1) 1 (1) 0.04
Keratin, type I cuticular Ha8 OS=Homo sapiens GN=KRT38 PE=2 SV=3
KT33B_HUMAN 31 47325 1 (1) 1 (1) 0.05
Keratin, type I cuticular Ha3-II OS=Homo sapiens GN=KRT33B PE=2 SV=3

+4 peptide matches (4 non-duplicate, 0 duplicate)


+7

Accession Score Description
1 GLND_ECOLI 31 Bifunctional uridylyltransferase/uridylyl-removing enzyme OS=Escherichia coli (strain K12) GN=glnD PE=1 SV=2

+8

Accession Score Description
1 EFTU1_ECOLI 30 Elongation factor Tu 1 OS=Escherichia coli (strain K12) GN=tufA PE=1 SV=1

+9

Accession Score Description
1 KPYK2_ECOLI 29 Pyruvate kinase II OS=Escherichia coli (strain K12) GN=pykA PE=1 SV=3

+10

Accession Score Description
1 MSCM_ECOLI 27 Miniconductance mechanosensitive channel MscM OS=Escherichia coli (strain K12) GN=mscM PE=1 SV=3
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