MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Andy2 |
| MS data file | : | PRT1311_JBEI_2.mgf |
| Database | : | Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues) |
| Timestamp | : | 8 Sep 2025 at 17:18:13 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,460 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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1| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88JG6_PSEPK | 2626 | histidine kinase OS=Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) OX=160488 GN=PP_2683 PE=4 SV=2 |
2| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TRYP_PIG | 225 | Trypsin OS=Sus scrofa PE=1 SV=1 |
3| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q70I01_9ACTO | 193 | Borrelidin polyketide synthase, type I OS=Streptomyces parvulus GN=borA2 PE=4 SV=1 |
4| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CH60_ECOLI | 102 | 60 kDa chaperonin OS=Escherichia coli (strain K12) GN=groL PE=1 SV=2 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 4.1 | CH60_ECOLI |
102 | 57464 | 20 (13) | 17 (11) | 0.60 | |
| 60 kDa chaperonin OS=Escherichia coli (strain K12) GN=groL PE=1 SV=2 | |||||||
20 peptide matches (19 non-duplicate, 1 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
| 3125 |
301.6857 | 601.3568 | 601.3547 | 3.51 | 0 | 10 | 0.35 | 1Score > 22 indicates identity Score > 18 indicates homology |
U | K.ISNIR.E | |
| 3137 |
307.6990 | 613.3834 | 613.3799 | 5.76 | 0 | 23 | 0.018 | 1Score > 18 indicates identity |
U | K.VTLGPK.G | |
| 3219 |
344.2070 | 686.3994 | 686.3963 | 4.61 | 0 | 13 | 0.23 | 1Score > 22 indicates identity Score > 19 indicates homology |
U | R.NVVLDK.S | |
| 3230 |
352.1919 | 702.3692 | 702.3661 | 4.54 | 0 | 16 | 0.034 | 1Score > 22 indicates identity Score > 14 indicates homology |
U | K.DGVSVAR.E | |
| 3234 |
360.1796 | 718.3446 | 718.3398 | 6.69 | 0 | 25 | 0.015 | 1Score > 22 indicates identity Score > 19 indicates homology |
U | K.APGFGDR.R | |
| 3274 |
402.2102 | 802.4058 | 802.4007 | 6.42 | 1 | 26 | 0.0087 | 1Score > 23 indicates identity Score > 18 indicates homology |
U | R.AMEAPLR.Q + Oxidation (M) | |
| 3299 |
414.2738 | 826.5330 | 826.5276 | 6.55 | 0 | 19 | 0.025 | 1Score > 16 indicates identity |
U | K.LAGGVAVIK.V | |
| 3345 |
453.7366 | 905.4586 | 905.4528 | 6.47 | 1 | 18 | 0.072 | 1Score > 22 indicates identity Score > 19 indicates homology |
U | K.LIAEAMDK.V + Oxidation (M) | |
| 3348 |
461.2590 | 920.5034 | 920.4967 | 7.29 | 0 | 19 | 0.053 | 1Score > 19 indicates identity |
U | K.SFGAPTITK.D | |
| 3411 |
1 |
493.2907 | 984.5668 | 984.5604 | 6.56 | 0 | 21 | 0.044 | 1Score > 20 indicates identity |
U | R.GVNVLADAVK.V |
| 3436 |
337.8470 | 1010.5192 | 1010.5145 | 4.63 | 1 | 15 | 0.14 | 1Score > 19 indicates identity |
U | R.VEDALHATR.A | |
D:\Xcalibur\Data\Jennifer\PRT1311 Andy\PRT1311_JBEI_2.rawScore > 19 indicates identity |
|||||||||||
| 3437 |
506.2676 | 1010.5206 | 1010.5145 | 6.09 | 1 | 30 | 0.0046 | 1Score > 19 indicates identity |
U | R.VEDALHATR.A | |
| 17.2 | 1 | 2 | 3.3 | 2 | VQEDGPPIR + Deamidated (NQ) | ||||||
| 3463 |
523.2833 | 1044.5520 | 1044.5451 | 6.63 | 1 | 27 | 0.017 | 1Score > 22 indicates identity |
U | K.ATLEDLGQAK.R | |
| 3478 |
532.2617 | 1062.5088 | 1062.5016 | 6.86 | 0 | 12 | 0.39 | 1Score > 21 indicates identity Score > 21 indicates homology |
U | K.ALSVPCSDSK.A | |
| 3573 |
601.2979 | 1200.5812 | 1200.5735 | 6.48 | 1 | 23 | 0.027 | 1Score > 20 indicates identity |
U | R.GQNEDQNVGIK.V | |
| 3720 |
469.2386 | 1404.6940 | 1404.6853 | 6.15 | 1 | 18 | 0.093 | 1Score > 20 indicates identity |
U | K.AVAAGMNPMDLKR.G + 2 Oxidation (M) | |
| 3870 |
587.6416 | 1759.9030 | 1759.8952 | 4.41 | 1 | 23 | 0.028 | 1Score > 20 indicates identity |
U | K.AIAQVGTISANSDETVGK.L | |
| 3871 |
880.9598 | 1759.9050 | 1759.8952 | 5.59 | 1 | 41 | 0.00021 | 1Score > 20 indicates identity Score > 16 indicates homology |
U | K.AIAQVGTISANSDETVGK.L | |
| 4150 |
801.4230 | 2401.2472 | 2401.2336 | 5.63 | 1 | 28 | 0.0043 | 1Score > 17 indicates identity |
U | K.ANDAAGDGTTTATVLAQAIITEGLK.A | |
| Score | Mass | Subset of | |||
|---|---|---|---|---|---|
| CH60_OLEAN |
41 | 56893 | 4.1 | ||
| 60 kDa chaperonin OS=Oleispira antarctica GN=groL PE=3 SV=1 | |||||
5| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88JG7_PSEPK | 42 | Fe-containing alcohol dehydrogenase OS=Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) OX=160488 GN=yiaY PE=3 SV=1 |
6| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | K1C10_HUMAN | 31 | Keratin, type I cytoskeletal 10 OS=Homo sapiens GN=KRT10 PE=1 SV=6 |
7| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | GLND_ECOLI | 31 | Bifunctional uridylyltransferase/uridylyl-removing enzyme OS=Escherichia coli (strain K12) GN=glnD PE=1 SV=2 |
8| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EFTU1_ECOLI | 30 | Elongation factor Tu 1 OS=Escherichia coli (strain K12) GN=tufA PE=1 SV=1 |
9| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | KPYK2_ECOLI | 29 | Pyruvate kinase II OS=Escherichia coli (strain K12) GN=pykA PE=1 SV=3 |
10| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | MSCM_ECOLI | 27 | Miniconductance mechanosensitive channel MscM OS=Escherichia coli (strain K12) GN=mscM PE=1 SV=3 |
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