MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Andy2 |
| MS data file | : | PRT1311_JBEI_2.mgf |
| Database | : | Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues) |
| Timestamp | : | 8 Sep 2025 at 17:18:13 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,460 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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1| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88JG6_PSEPK | 2626 | histidine kinase OS=Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) OX=160488 GN=PP_2683 PE=4 SV=2 |
2| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TRYP_PIG | 225 | Trypsin OS=Sus scrofa PE=1 SV=1 |
3| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q70I01_9ACTO | 193 | Borrelidin polyketide synthase, type I OS=Streptomyces parvulus GN=borA2 PE=4 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 3.1 | Q70I01_9ACTO |
193 | 166730 | 15 (10) | 7 (5) | 0.13 | |
| Borrelidin polyketide synthase, type I OS=Streptomyces parvulus GN=borA2 PE=4 SV=1 | |||||||
15 peptide matches (13 non-duplicate, 2 duplicate)| Score | Mass | Subset of | |||
|---|---|---|---|---|---|
| K4I6L4_ACP-AT-9ACTN |
144 | 263142 | 3.1 | ||
| Flv003-ACP-AT-FluC OS=Actinomadura vulgaris subsp. lanata OX=1233072 GN=fluC PE=4 SV=1 | |||||
| A4F7N8_SACEN |
17 | 372029 | 3.1 | ||
| EryAI Erythromycin polyketide synthase modules 1 and 2 OS=Saccharopolyspora erythraea (strain ATCC 11635 / DSM 40517 / JCM 4748 / NBRC 13426 / NCIMB 8594 / NRRL 2338) GN=eryAI PE=4 SV=1 | |||||
| 5 samesets of A4F7N8_SACEN | |||||
| DebsM6(epoAT4)_KRnull |
17 | 147321 | |||
| description | |||||
| DEBSM2TE |
17 | 186551 | |||
| DEBSM2TE | |||||
| ERYA3_SACER |
17 | 178674 | |||
| 6-deoxyerythronolide-B synthase EryA3, modules 5 and 6 OS=Saccharopolyspora erythraea GN=eryA PE=1 SV=4 | |||||
| A4F7P1_SACEN |
17 | 333724 | |||
| EryAIII Erythromycin polyketide synthase modules 5 and 6 OS=Saccharopolyspora erythraea (strain ATCC 11635 / DSM 40517 / JCM 4748 / NBRC 13426 / NCIMB 8594 / NRRL 2338) GN=eryAIII PE=4 SV=1 | |||||
| ERYA1_SACER |
17 | 366515 | |||
| 6-deoxyerythronolide-B synthase EryA1, modules 1 and 2 OS=Saccharopolyspora erythraea GN=eryA PE=1 SV=1 | |||||
4| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CH60_ECOLI | 102 | 60 kDa chaperonin OS=Escherichia coli (strain K12) GN=groL PE=1 SV=2 |
5| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88JG7_PSEPK | 42 | Fe-containing alcohol dehydrogenase OS=Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) OX=160488 GN=yiaY PE=3 SV=1 |
6| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | K1C10_HUMAN | 31 | Keratin, type I cytoskeletal 10 OS=Homo sapiens GN=KRT10 PE=1 SV=6 |
7| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | GLND_ECOLI | 31 | Bifunctional uridylyltransferase/uridylyl-removing enzyme OS=Escherichia coli (strain K12) GN=glnD PE=1 SV=2 |
8| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EFTU1_ECOLI | 30 | Elongation factor Tu 1 OS=Escherichia coli (strain K12) GN=tufA PE=1 SV=1 |
9| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | KPYK2_ECOLI | 29 | Pyruvate kinase II OS=Escherichia coli (strain K12) GN=pykA PE=1 SV=3 |
10| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | MSCM_ECOLI | 27 | Miniconductance mechanosensitive channel MscM OS=Escherichia coli (strain K12) GN=mscM PE=1 SV=3 |
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