MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Andy2 |
| MS data file | : | PRT1311_JBEI_2.mgf |
| Database | : | Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues) |
| Timestamp | : | 8 Sep 2025 at 17:18:13 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,460 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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11| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | UGPC_ECOLI | 25 | sn-glycerol-3-phosphate import ATP-binding protein UgpC OS=Escherichia coli (strain K12) GN=ugpC PE=1 SV=3 |
12| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | WECD_ECOLI | 25 | dTDP-fucosamine acetyltransferase OS=Escherichia coli (strain K12) GN=wecD PE=3 SV=3 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 12.1 | WECD_ECOLI |
25 | 24262 | 1 (1) | 1 (1) | 0.10 | |
| dTDP-fucosamine acetyltransferase OS=Escherichia coli (strain K12) GN=wecD PE=3 SV=3 | |||||||
1 peptide matches (1 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 3225 |
350.2305 | 698.4464 | 698.4439 | 3.66 | 0 | 25 | 0.006 | 1Score > 15 indicates identity |
U | R.IGLLAGR.G |
13| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TGT_ECOLI | 25 | description |
14| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | A4XUL5_PSEMY | 23 | Catalase OS=Pseudomonas mendocina (strain ymp) GN=Pmen_2273 PE=3 SV=1 |
15| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | GLTB_ECOLI | 22 | Glutamate synthase [NADPH] large chain OS=Escherichia coli (strain K12) GN=gltB PE=1 SV=3 |
16| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | GLPD_ECOLI | 21 | Aerobic glycerol-3-phosphate dehydrogenase OS=Escherichia coli (strain K12) GN=glpD PE=1 SV=3 |
17| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | NIKA_ECOLI | 21 | description |
18| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | OPGG_ECOLI | 21 | Glucans biosynthesis protein G OS=Escherichia coli (strain K12) GN=mdoG PE=1 SV=1 |
19| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | FTSW_ECOLI | 15 | Lipid II flippase FtsW OS=Escherichia coli (strain K12) GN=ftsW PE=1 SV=1 |
20| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | GLTP_ECOLI | 14 | description |
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