MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2
MS data file : PRT1311_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:18:13 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,460

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 11–20 (out of 20)

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-11

Accession Score Description
1 UGPC_ECOLI 25 sn-glycerol-3-phosphate import ATP-binding protein UgpC OS=Escherichia coli (strain K12) GN=ugpC PE=1 SV=3
Score Mass Matches Sequences emPAI
11.1 UGPC_ECOLI 25 39556 2 (1) 2 (1) 0.06
sn-glycerol-3-phosphate import ATP-binding protein UgpC OS=Escherichia coli (strain K12) GN=ugpC PE=1 SV=3
1 sameset of UGPC_ECOLI
MODF_ECOLI 25 54730 3 (1) 3 (1) 0.04
Putative molybdenum transport ATP-binding protein ModF OS=Escherichia coli (strain K12) GN=modF PE=3 SV=2

-2 peptide matches (2 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
3116   589.3699 588.3626 588.3595 5.31 0 6 0.78 +8Score > 17 indicates identity K.STLLR.M
3252   746.3729 745.3656 745.3606 6.75 1 25 0.023 +1Score > 21 indicates identity U K.QQIAER.V + 2 Deamidated (NQ)

+12

Accession Score Description
1 WECD_ECOLI 25 dTDP-fucosamine acetyltransferase OS=Escherichia coli (strain K12) GN=wecD PE=3 SV=3

+13

Accession Score Description
1 TGT_ECOLI 25 description

+14

Accession Score Description
1 A4XUL5_PSEMY 23 Catalase OS=Pseudomonas mendocina (strain ymp) GN=Pmen_2273 PE=3 SV=1

+15

Accession Score Description
1 GLTB_ECOLI 22 Glutamate synthase [NADPH] large chain OS=Escherichia coli (strain K12) GN=gltB PE=1 SV=3

+16

Accession Score Description
1 GLPD_ECOLI 21 Aerobic glycerol-3-phosphate dehydrogenase OS=Escherichia coli (strain K12) GN=glpD PE=1 SV=3

+17

Accession Score Description
1 NIKA_ECOLI 21 description

+18

Accession Score Description
1 OPGG_ECOLI 21 Glucans biosynthesis protein G OS=Escherichia coli (strain K12) GN=mdoG PE=1 SV=1

+19

Accession Score Description
1 FTSW_ECOLI 15 Lipid II flippase FtsW OS=Escherichia coli (strain K12) GN=ftsW PE=1 SV=1

+20

Accession Score Description
1 GLTP_ECOLI 14 description
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