MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2
MS data file : PRT1311_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:18:13 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,460

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 20)

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-1

Accession Score Description
1 Q88JG6_PSEPK 2626 histidine kinase OS=Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) OX=160488 GN=PP_2683 PE=4 SV=2
Score Mass Matches Sequences emPAI
1.1 Q88JG6_PSEPK 2626 61597 122 (98) 25 (22) 2.45
histidine kinase OS=Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) OX=160488 GN=PP_2683 PE=4 SV=2

-122 peptide matches (44 non-duplicate, 78 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
3124   601.3705 600.3632 600.3595 6.23 1 28 0.021 +1Score > 23 indicates identity U E.LLEAR.D
3145 +2 309.6730 617.3314 617.3285 4.72 0 20 0.069 +1Score > 21 indicates identity U R.SWLGR.G
3153 +3 311.1913 620.3680 620.3646 5.59 0 15 0.049 +1Score > 14 indicates identity U K.ILGYR.I
3181 +4 321.7197 641.4248 641.4224 3.78 0 30 0.0017 +1Score > 15 indicates identity U R.ILLGAR.R
3204   330.1784 658.3422 658.3398 3.68 0 11 0.28 +1Score > 22 indicates identity
Score > 18 indicates homology		 U R.GIAQDR.L
3244   733.3654 732.3581 732.3555 3.58 0 16 0.21 +1Score > 22 indicates identity U E.VWDTGR.G
3265   783.4513 782.4440 782.4398 5.33 0 16 0.051 +1Score > 15 indicates identity U R.AANPALAR.M
3266 +3 392.2294 782.4442 782.4398 5.62 0 48 3e-005 +1Score > 15 indicates identity U R.AANPALAR.M
3286 +5 408.2734 814.5322 814.5276 5.68 0 49 1.4e-005 +1Score > 13 indicates identity U R.LLISTLR.E
3398   975.4913 974.4840 974.4821 1.93 1 5 1 +1Score > 22 indicates identity
Score > 18 indicates homology		 U R.LEVWDTGR.G
3402 +4 488.2513 974.4880 974.4821 6.06 1 61 6.2e-006 +1Score > 21 indicates identity U R.LEVWDTGR.G
3409 +2 491.2356 980.4566 980.4498 7.00 0 53 2.5e-005 +1Score > 20 indicates identity U R.NFLSNACR.Y
3446 +4 514.3008 1026.5870 1026.5822 4.75 1 69 4.5e-007 +1Score > 18 indicates identity U R.EGALLALQGR.A
3471 +2 530.2669 1058.5192 1058.5145 4.50 1 35 0.0022 +1Score > 21 indicates identity U K.SHYPELSAR.L
3474 +2 353.8477 1058.5213 1058.5145 6.42 1 26 0.016 +1Score > 20 indicates identity U K.SHYPELSAR.L
3493 +7 542.3333 1082.6520 1082.6448 6.70 1 75 5.5e-008 +1Score > 15 indicates identity U K.GVGLGLAIVER.I
3506   1092.6831 1091.6758 1091.6703 5.08 0 18 0.016 +1Score > 13 indicates identity U R.AVLSQLLLVH.-
3508 +4 546.8456 1091.6766 1091.6703 5.84 0 63 4.6e-007 +1Score > 13 indicates identity U R.AVLSQLLLVH.-
3603 +4 616.8514 1231.6882 1231.6812 5.69 1 56 1.1e-005 +1Score > 19 indicates identity U R.GSVFSIEVPLGK.E
3613 +4 619.3267 1236.6388 1236.6350 3.12 0 81 4.9e-008 +1Score > 20 indicates identity U R.IADYAISTDLR.L
3688 +4 450.9226 1349.7460 1349.7415 3.29 0 56 7.2e-006 +1Score > 17 indicates identity U R.ALAGLLGLGDHSAR.K
3689 +3 675.8812 1349.7478 1349.7415 4.68 0 74 9e-008 +1Score > 16 indicates identity U R.ALAGLLGLGDHSAR.K
3736 +3 358.7325 1430.9009 1430.8973 2.52 0 39 0.00013 +1Score > 13 indicates identity U K.LGAPLLNKPVKPGK.L
3739 +2 477.9753 1430.9041 1430.8973 4.74 0 54 4e-006 +1Score > 13 indicates identity U K.LGAPLLNKPVKPGK.L
3740   716.4595 1430.9044 1430.8973 5.00 0 34 0.00036 +1Score > 13 indicates identity U K.LGAPLLNKPVKPGK.L
3759 +3 370.4686 1477.8453 1477.8365 5.96 1 25 0.0034 +1Score > 13 indicates identity U R.ALAGLLGLGDHSARK.S
3761 +1 493.6226 1477.8460 1477.8365 6.42 1 38 0.00018 +1Score > 13 indicates identity U R.ALAGLLGLGDHSARK.S
3784   772.9279 1543.8412 1543.8358 3.51 0 38 0.00051 +1Score > 18 indicates identity U R.LDQAAVKPDVAVYR.L
3786 +3 515.6213 1543.8421 1543.8358 4.04 0 77 6.2e-008 +1Score > 18 indicates identity U R.LDQAAVKPDVAVYR.L
3789   773.4296 1544.8446 1544.8198 16.1 0 53 1.7e-005 +1Score > 18 indicates identity U R.LDQAAVKPDVAVYR.L + Deamidated (NQ)
3844 +1 862.9745 1723.9344 1723.9257 5.07 1 52 1.7e-005 +1Score > 17 indicates identity U R.EHFATAIPAVIITADR.S
3888 +1 903.9769 1805.9392 1805.9312 4.46 1 27 0.008 +1Score > 18 indicates identity U R.LQDIFLEFNQLDVGR.A
3890   603.3208 1806.9406 1806.9152 14.0 1 20 0.048 +1Score > 19 indicates identity U R.LQDIFLEFNQLDVGR.A + Deamidated (NQ)
3892 +1 904.4794 1806.9442 1806.9152 16.1 1 78 7e-008 +1Score > 19 indicates identity U R.LQDIFLEFNQLDVGR.A + Deamidated (NQ)
D:\Xcalibur\Data\Jennifer\PRT1311 Andy\PRT1311_JBEI_2.raw

Score > 19 indicates identity
3893   603.3221 1806.9445 1806.9152 16.2 1 46 0.0001 -1Score > 19 indicates identity U R.LQDIFLEFNQLDVGR.A + Deamidated (NQ)
16.2 1 38 0.00067 2 R.LQDIFLEFNQLDVGR.A + Deamidated (NQ)
16.2 1 31 0.003 3 R.LQDIFLEFNQLDVGR.A + Deamidated (NQ)
19.8 0 4 1.7 4 QIGMIFQHFNLLSSR   + Deamidated (NQ); Oxidation (M)
19.8 0 4 1.7 4 QIGMIFQHFNLLSSR   + Deamidated (NQ); Oxidation (M)
4.50 1 0 3.6 6 SSRSAPLYDTIVIVTGE  
3902   608.6671 1822.9795 1822.9689 5.78 0 12 0.13 +1Score > 16 indicates identity U K.YLAAASHDLLQPLNAAR.L
3905   912.9974 1823.9802 1823.9529 15.0 0 4 1.1 +1Score > 17 indicates identity U K.YLAAASHDLLQPLNAAR.L + Deamidated (NQ)
3906 +1 609.0013 1823.9821 1823.9529 16.0 0 40 0.00032 +1Score > 17 indicates identity U K.YLAAASHDLLQPLNAAR.L + Deamidated (NQ)
3907   609.0017 1823.9833 1823.9529 16.6 0 4 1.1 +1Score > 17 indicates identity U K.YLAAASHDLLQPLNAAR.L + Deamidated (NQ)
3974   689.7087 2066.1043 2066.0908 6.50 1 36 0.00084 +1Score > 17 indicates identity U K.DKYLAAASHDLLQPLNAAR.L
3977   690.0430 2067.1072 2067.0748 15.6 1 14 0.096 +1Score > 17 indicates identity U K.DKYLAAASHDLLQPLNAAR.L + Deamidated (NQ)
3978 +2 517.7841 2067.1073 2067.0748 15.7 1 28 0.0044 +1Score > 17 indicates identity U K.DKYLAAASHDLLQPLNAAR.L + Deamidated (NQ)
4064   1125.1323 2248.2500 2248.2216 12.7 1 6 0.34 +1Score > 13 indicates identity U K.EVPLAVHQAVPLPSVGDPLPGR.R + Deamidated (NQ)
4065 +3 750.4243 2248.2511 2248.2216 13.1 1 45 3.9e-005 +1Score > 13 indicates identity U K.EVPLAVHQAVPLPSVGDPLPGR.R + Deamidated (NQ)

2 subsets and intersections (8 subset proteins in total)

Score Mass Subset of
CYSM_ECOLI 42 32758 1.1
Cysteine synthase B OS=Escherichia coli (strain K12) GN=cysM PE=1 SV=1
ATMA_ECOLI 28 0 1.1
description
6 samesets of ATMA_ECOLI
SGBU_ECOLI 28 32662
Putative L-ribulose-5-phosphate 3-epimerase SgbU OS=Escherichia coli (strain K12) GN=sgbU PE=3 SV=2
EXUR_ECOLI 28 0
description
MDTQ_ECOLI 28 0
description
SPEE_ECOLI 28 0
description
UXUR_ECOLI 28 0
description
Q8S7V0_ORYSJ 28 0
description

+2

Accession Score Description
1 TRYP_PIG 225 Trypsin OS=Sus scrofa PE=1 SV=1

+3

Accession Score Description
1 Q70I01_9ACTO 193 Borrelidin polyketide synthase, type I OS=Streptomyces parvulus GN=borA2 PE=4 SV=1

+4

Accession Score Description
1 CH60_ECOLI 102 60 kDa chaperonin OS=Escherichia coli (strain K12) GN=groL PE=1 SV=2

+5

Accession Score Description
1 Q88JG7_PSEPK 42 Fe-containing alcohol dehydrogenase OS=Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) OX=160488 GN=yiaY PE=3 SV=1

+6

Accession Score Description
1 K1C10_HUMAN 31 Keratin, type I cytoskeletal 10 OS=Homo sapiens GN=KRT10 PE=1 SV=6

+7

Accession Score Description
1 GLND_ECOLI 31 Bifunctional uridylyltransferase/uridylyl-removing enzyme OS=Escherichia coli (strain K12) GN=glnD PE=1 SV=2

+8

Accession Score Description
1 EFTU1_ECOLI 30 Elongation factor Tu 1 OS=Escherichia coli (strain K12) GN=tufA PE=1 SV=1

+9

Accession Score Description
1 KPYK2_ECOLI 29 Pyruvate kinase II OS=Escherichia coli (strain K12) GN=pykA PE=1 SV=3

+10

Accession Score Description
1 MSCM_ECOLI 27 Miniconductance mechanosensitive channel MscM OS=Escherichia coli (strain K12) GN=mscM PE=1 SV=3
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