MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2
MS data file : PRT1311_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:18:13 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,460

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 20)

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+1

Accession Score Description
1 Q88JG6_PSEPK 2626 histidine kinase OS=Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) OX=160488 GN=PP_2683 PE=4 SV=2

+2

Accession Score Description
1 TRYP_PIG 225 Trypsin OS=Sus scrofa PE=1 SV=1

-3

Accession Score Description
1 Q70I01_9ACTO 193 Borrelidin polyketide synthase, type I OS=Streptomyces parvulus GN=borA2 PE=4 SV=1
Score Mass Matches Sequences emPAI
3.1 Q70I01_9ACTO 193 166730 15 (10) 7 (5) 0.13
Borrelidin polyketide synthase, type I OS=Streptomyces parvulus GN=borA2 PE=4 SV=1

-15 peptide matches (13 non-duplicate, 2 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
3305   421.7597 841.5048 841.4882 19.7 1 0 4.1 +5Score > 19 indicates identity U E.VAQGRGVR.G
3793   522.2962 1563.8668 1563.8369 19.1 0 17 0.044 +1Score > 16 indicates identity U K.SNIGHAQAAAGVAGVIK.M + Deamidated (NQ)
3825   563.3010 1686.8812 1686.8941 -7.64 1 2 1.1 +2Score > 20 indicates identity
Score > 15 indicates homology		 R.LGDPIEAQALLATYGR.G
3931   947.0344 1892.0542 1892.0196 18.3 1 19 0.017 +1Score > 14 indicates identity U R.TVWAQAGLFALEVALFR.L + Deamidated (NQ)
3932   631.6927 1892.0563 1892.0196 19.4 1 43 8.4e-005 +1Score > 14 indicates identity U R.TVWAQAGLFALEVALFR.L + Deamidated (NQ)
3948   977.4958 1952.9770 1952.9705 3.37 0 64 1.1e-006 +1Score > 20 indicates identity
Score > 17 indicates homology		 U R.GVASSASGGLAFVFAGQGSQR.L
3949   651.9999 1952.9779 1952.9705 3.80 0 15 0.16 +1Score > 20 indicates identity U R.GVASSASGGLAFVFAGQGSQR.L
3951   652.3353 1953.9841 1953.9545 15.2 0 42 0.00014 +1Score > 20 indicates identity
Score > 16 indicates homology		 U R.GVASSASGGLAFVFAGQGSQR.L + Deamidated (NQ)
4002   1080.0187 2158.0228 2158.0120 5.03 1 41 0.00038 +1Score > 19 indicates identity U R.EGGFLDGAGQFDAAFFGISPR.E
4003 +1 720.3484 2158.0234 2158.0120 5.27 1 23 0.024 +1Score > 19 indicates identity U R.EGGFLDGAGQFDAAFFGISPR.E
4006   720.6829 2159.0269 2158.9960 14.3 1 40 0.00049 +1Score > 19 indicates identity U R.EGGFLDGAGQFDAAFFGISPR.E + Deamidated (NQ)
4243 -1 866.1343 2595.3811 2595.3486 12.5 0 29 0.0025 +1Score > 16 indicates identity U R.TVLSAAASLYVQGHPVDWAPLFPR.A + Deamidated (NQ)
4242   866.1342 2595.3808 2595.3486 12.4 0 (25) 0.0074 +1Score > 16 indicates identity U R.TVLSAAASLYVQGHPVDWAPLFPR.A + Deamidated (NQ)
4244   649.8531 2595.3833 2595.3486 13.4 0 7 0.38 +1Score > 16 indicates identity U R.TVLSAAASLYVQGHPVDWAPLFPR.A + Deamidated (NQ)

2 subsets and intersections (7 subset proteins in total)

Score Mass Subset of
K4I6L4_ACP-AT-9ACTN 144 263142 3.1
Flv003-ACP-AT-FluC OS=Actinomadura vulgaris subsp. lanata OX=1233072 GN=fluC PE=4 SV=1
A4F7N8_SACEN 17 372029 3.1
EryAI Erythromycin polyketide synthase modules 1 and 2 OS=Saccharopolyspora erythraea (strain ATCC 11635 / DSM 40517 / JCM 4748 / NBRC 13426 / NCIMB 8594 / NRRL 2338) GN=eryAI PE=4 SV=1
5 samesets of A4F7N8_SACEN
DebsM6(epoAT4)_KRnull 17 147321
description
DEBSM2TE 17 186551
DEBSM2TE
ERYA3_SACER 17 178674
6-deoxyerythronolide-B synthase EryA3, modules 5 and 6 OS=Saccharopolyspora erythraea GN=eryA PE=1 SV=4
A4F7P1_SACEN 17 333724
EryAIII Erythromycin polyketide synthase modules 5 and 6 OS=Saccharopolyspora erythraea (strain ATCC 11635 / DSM 40517 / JCM 4748 / NBRC 13426 / NCIMB 8594 / NRRL 2338) GN=eryAIII PE=4 SV=1
ERYA1_SACER 17 366515
6-deoxyerythronolide-B synthase EryA1, modules 1 and 2 OS=Saccharopolyspora erythraea GN=eryA PE=1 SV=1

+4

Accession Score Description
1 CH60_ECOLI 102 60 kDa chaperonin OS=Escherichia coli (strain K12) GN=groL PE=1 SV=2

+5

Accession Score Description
1 Q88JG7_PSEPK 42 Fe-containing alcohol dehydrogenase OS=Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) OX=160488 GN=yiaY PE=3 SV=1

+6

Accession Score Description
1 K1C10_HUMAN 31 Keratin, type I cytoskeletal 10 OS=Homo sapiens GN=KRT10 PE=1 SV=6

+7

Accession Score Description
1 GLND_ECOLI 31 Bifunctional uridylyltransferase/uridylyl-removing enzyme OS=Escherichia coli (strain K12) GN=glnD PE=1 SV=2

+8

Accession Score Description
1 EFTU1_ECOLI 30 Elongation factor Tu 1 OS=Escherichia coli (strain K12) GN=tufA PE=1 SV=1

+9

Accession Score Description
1 KPYK2_ECOLI 29 Pyruvate kinase II OS=Escherichia coli (strain K12) GN=pykA PE=1 SV=3

+10

Accession Score Description
1 MSCM_ECOLI 27 Miniconductance mechanosensitive channel MscM OS=Escherichia coli (strain K12) GN=mscM PE=1 SV=3
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