MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy1
MS data file : PRT1311_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:17:19 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,450

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 801–900 (out of 4217)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
561 320.9017 319.8944
562 320.9018 319.8945
563 320.9018 319.8945
564 320.9018 319.8945
565 320.9018 319.8945
566 320.9019 319.8946
567 320.9020 319.8947
568 320.9020 319.8947
569 320.9020 319.8947
570 320.9021 319.8948
571 320.9026 319.8953
572 321.0162 320.0089
573 321.0934 320.0861
574 321.0939 320.0866
575 321.0943 320.0870
576 321.0949 320.0876
577 321.0949 320.0876
578 321.0951 320.0878
579 321.0953 320.0880
580 321.0953 320.0880
581 321.1295 320.1222
582 321.1306 320.1233
583 321.1321 320.1248
584 321.1324 320.1251
585 321.1324 320.1251
586 321.1325 320.1252
587 321.1325 320.1252
588 321.1325 320.1252
589 321.1325 320.1252
590 321.1326 320.1253
591 321.1326 320.1253
592 321.1326 320.1253
593 321.1326 320.1253
594 321.1327 320.1254
595 321.1327 320.1254
596 321.1327 320.1254
597 321.1327 320.1254
598 321.1327 320.1254
599 321.1328 320.1255
600 321.1330 320.1257
601 321.1692 320.1619
602 321.1693 320.1620
603 321.1693 320.1620
604 321.1693 320.1620
605 321.1693 320.1620
606 321.1694 320.1621
607 321.1694 320.1621
608 321.1695 320.1622
609 321.1695 320.1622
610 321.1696 320.1623
611 321.1696 320.1623
612 321.1696 320.1623
613 321.1697 320.1624
614 321.1697 320.1624
615 321.1699 320.1626
616 321.1700 320.1627
617 321.1700 320.1627
618 321.1700 320.1627
619 321.1701 320.1628
620 321.1701 320.1628
621 321.1704 320.1631
622 321.1705 320.1632
623 321.1705 320.1632
624 321.2054 320.1981
625 321.2057 320.1984
626 321.2057 320.1984
627 321.2057 320.1984
628 321.2058 320.1985
629 321.2060 320.1987
630 321.2062 320.1989
631 321.2421 320.2348
632 321.2422 320.2349
633 321.2422 320.2349
634 321.2426 320.2353
635 321.2631 320.2558
636 321.9749 320.9676
637 322.0004 320.9931
638 322.1888 321.1815
639 322.1889 321.1816
640 322.2469 321.2396
641 322.9830 321.9757
642 322.9833 321.9760
643 322.9834 321.9761
644 323.1094 322.1021
645 323.1106 322.1033
646 323.1107 322.1034
647 323.1107 322.1034
648 323.1111 322.1038
649 323.1111 322.1038
650 323.1113 322.1040
651 323.1116 322.1043
652 323.1117 322.1044
653 323.1118 322.1045
654 323.1119 322.1046
655 323.1119 322.1046
656 323.1472 322.1399
657 323.1478 322.1405
658 323.1482 322.1409
659 323.1483 322.1410
660 323.1483 322.1410
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