MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy1
MS data file : PRT1311_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:17:19 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,450

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 701–800 (out of 4217)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
461 317.1740 316.1667
462 317.1740 316.1667
463 317.1742 316.1669
464 317.1743 316.1670
465 317.1744 316.1671
466 317.1744 316.1671
467 317.1744 316.1671
468 317.1746 316.1673
469 317.1747 316.1674
470 317.1747 316.1674
471 317.1758 316.1685
472 318.1923 317.1850
473 318.1923 317.1850
474 318.1925 317.1852
475 318.1926 317.1853
476 318.1926 317.1853
477 318.1927 317.1854
478 318.1927 317.1854
479 318.1927 317.1854
480 318.1928 317.1855
481 318.1929 317.1856
482 319.0402 318.0329
483 319.0408 318.0335
484 319.0782 318.0709
485 319.1143 318.1070
486 319.1145 318.1072
487 319.1161 318.1088
488 319.1163 318.1090
489 319.1164 318.1091
490 319.1165 318.1092
491 319.1166 318.1093
492 319.1166 318.1093
493 319.1166 318.1093
494 319.1167 318.1094
495 319.1167 318.1094
496 319.1167 318.1094
497 319.1168 318.1095
498 319.1168 318.1095
499 319.1168 318.1095
500 319.1168 318.1095
501 319.1171 318.1098
502 319.1538 318.1465
503 319.1538 318.1465
504 319.1538 318.1465
505 319.1539 318.1466
506 319.1540 318.1467
507 319.1540 318.1467
508 319.1540 318.1467
509 319.1540 318.1467
510 319.1541 318.1468
511 319.1541 318.1468
512 319.1541 318.1468
513 319.1542 318.1469
514 319.1542 318.1469
515 319.1545 318.1472
516 319.1545 318.1472
517 319.1545 318.1472
518 319.1545 318.1472
519 319.1548 318.1475
520 319.1549 318.1476
521 319.1671 318.1598
522 319.1672 318.1599
523 319.1672 318.1599
524 319.1674 318.1601
525 319.1898 318.1825
526 319.1899 318.1826
527 319.1900 318.1827
528 319.1901 318.1828
529 319.1902 318.1829
530 319.1902 318.1829
531 319.1903 318.1830
532 319.1904 318.1831
533 319.1904 318.1831
534 319.1910 318.1837
535 319.1912 318.1839
536 319.2262 318.2189
537 319.2262 318.2189
538 319.2263 318.2190
539 319.2263 318.2190
540 319.2264 318.2191
541 319.2266 318.2193
542 319.2266 318.2193
543 319.2269 318.2196
544 319.2275 318.2202
545 319.2477 318.2404
546 319.9947 318.9874
547 319.9949 318.9876
548 319.9950 318.9877
549 319.9950 318.9877
550 319.9951 318.9878
551 319.9951 318.9878
552 320.1713 319.1640
553 320.1719 319.1646
554 320.1720 319.1647
555 320.6278 319.6205
556 320.9015 319.8942
557 320.9016 319.8943
558 320.9016 319.8943
559 320.9016 319.8943
560 320.9017 319.8944
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