MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy1
MS data file : PRT1311_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:17:19 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,450

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 601–700 (out of 4217)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
361 313.0876 312.0803
362 313.0878 312.0805
363 313.0988 312.0915
364 313.1269 312.1196
365 313.1270 312.1197
366 313.1285 312.1212
367 313.1290 312.1217
368 313.1292 312.1219
369 313.1294 312.1221
370 313.1296 312.1223
371 313.1646 312.1573
372 313.1657 312.1584
373 313.1658 312.1585
374 313.1660 312.1587
375 313.1662 312.1589
376 313.1844 312.1771
377 313.1844 312.1771
378 313.1845 312.1772
379 313.1846 312.1773
380 313.1847 312.1774
381 313.1847 312.1774
382 313.1847 312.1774
383 313.1848 312.1775
384 313.1849 312.1776
385 313.1852 312.1779
386 313.2215 312.2142
387 313.2215 312.2142
388 313.2215 312.2142
389 313.2217 312.2144
390 313.2217 312.2144
391 313.2224 312.2151
392 314.0121 313.0048
393 315.0538 314.0465
394 315.1021 314.0948
395 315.1023 314.0950
396 315.1023 314.0950
397 315.1023 314.0950
398 315.1027 314.0954
399 315.1212 314.1139
400 315.1213 314.1140
401 315.1214 314.1141
402 315.1215 314.1142
403 315.1216 314.1143
404 315.1216 314.1143
405 315.1217 314.1144
406 315.1219 314.1146
407 315.1414 314.1341
408 315.1419 314.1346
409 315.1435 314.1362
410 315.1451 314.1378
411 315.1451 314.1378
412 315.1453 314.1380
413 315.1454 314.1381
414 315.1454 314.1381
415 315.1456 314.1383
416 315.1457 314.1384
417 315.1459 314.1386
418 315.1461 314.1388
419 315.1461 314.1388
420 315.1580 314.1507
421 315.1582 314.1509
422 315.1582 314.1509
423 315.1584 314.1511
424 315.1586 314.1513
425 315.1586 314.1513
426 315.1589 314.1516
427 315.1593 314.1520
428 315.1815 314.1742
429 315.1818 314.1745
430 315.1818 314.1745
431 316.1766 315.1693
432 316.1767 315.1694
433 317.0217 316.0144
434 317.0822 316.0749
435 317.0997 316.0924
436 317.0998 316.0925
437 317.0998 316.0925
438 317.0999 316.0926
439 317.0999 316.0926
440 317.0999 316.0926
441 317.0999 316.0926
442 317.1000 316.0927
443 317.1002 316.0929
444 317.1003 316.0930
445 317.1006 316.0933
446 317.1376 316.1303
447 317.1377 316.1304
448 317.1377 316.1304
449 317.1377 316.1304
450 317.1377 316.1304
451 317.1377 316.1304
452 317.1378 316.1305
453 317.1379 316.1306
454 317.1379 316.1306
455 317.1381 316.1308
456 317.1382 316.1309
457 317.1382 316.1309
458 317.1383 316.1310
459 317.1390 316.1317
460 317.1538 316.1465
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