MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy1
MS data file : PRT1311_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:17:19 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,450

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 501–600 (out of 4217)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
261 309.1695 308.1622
262 309.1696 308.1623
263 309.1696 308.1623
264 309.1696 308.1623
265 309.1696 308.1623
266 309.1696 308.1623
267 309.1696 308.1623
268 309.1696 308.1623
269 309.1700 308.1627
270 309.1700 308.1627
271 309.1702 308.1629
272 309.1708 308.1635
273 309.1884 308.1811
274 309.1885 308.1812
275 309.1886 308.1813
276 309.1887 308.1814
277 309.1888 308.1815
278 309.1890 308.1817
279 309.1891 308.1818
280 309.1893 308.1820
281 309.2064 308.1991
282 309.2068 308.1995
283 309.2070 308.1997
284 310.1118 309.1045
285 310.1121 309.1048
286 311.0566 310.0493
287 311.0568 310.0495
288 311.0714 310.0641
289 311.0717 310.0644
290 311.0909 310.0836
291 311.0909 310.0836
292 311.0910 310.0837
293 311.0911 310.0838
294 311.0911 310.0838
295 311.1100 310.1027
296 311.1111 310.1038
297 311.1112 310.1039
298 311.1112 310.1039
299 311.1114 310.1041
300 311.1114 310.1041
301 311.1116 310.1043
302 311.1118 310.1045
303 311.1118 310.1045
304 311.1120 310.1047
305 311.1479 310.1406
306 311.1484 310.1411
307 311.1484 310.1411
308 311.1484 310.1411
309 311.1485 310.1412
310 311.1486 310.1413
311 311.1486 310.1413
312 311.1487 310.1414
313 311.1489 310.1416
314 311.1489 310.1416
315 311.1489 310.1416
316 311.1490 310.1417
317 311.1490 310.1417
318 311.1491 310.1418
319 311.1492 310.1419
320 311.1665 310.1592
321 311.1668 310.1595
322 311.1668 310.1595
323 311.1669 310.1596
324 311.1670 310.1597
325 311.1670 310.1597
326 311.1671 310.1598
327 311.1672 310.1599
328 311.1673 310.1600
329 311.1848 310.1775
330 311.1851 310.1778
331 311.1852 310.1779
332 311.1854 310.1781
333 311.1856 310.1783
334 311.2054 310.1981
335 311.2054 310.1981
336 311.2055 310.1982
337 311.2055 310.1982
338 311.2056 310.1983
339 311.2057 310.1984
340 311.2061 310.1988
341 311.2062 310.1989
342 311.2230 310.2157
343 311.9456 310.9383
344 311.9460 310.9387
345 311.9461 310.9388
346 312.1319 311.1246
347 312.1322 311.1249
348 312.1322 311.1249
349 312.1322 311.1249
350 312.1324 311.1251
351 312.1324 311.1251
352 312.1324 311.1251
353 313.0277 312.0204
354 313.0277 312.0204
355 313.0865 312.0792
356 313.0873 312.0800
357 313.0874 312.0801
358 313.0874 312.0801
359 313.0876 312.0803
360 313.0876 312.0803
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